Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | EKI57_RS21645 | Genome accession | NZ_CP037943 |
| Coordinates | 4217798..4218238 (-) | Length | 146 a.a. |
| NCBI ID | WP_000360902.1 | Uniprot ID | A7ZHJ7 |
| Organism | Escherichia coli strain CFSAN027343 | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
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Genomic Context
Location: 4212798..4223238
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKI57_RS21625 (EKI57_21625) | coaE | 4213288..4213908 (-) | 621 | WP_001269520.1 | dephospho-CoA kinase | - |
| EKI57_RS30415 | - | 4213933..4213977 (+) | 45 | WP_120795372.1 | protein YacM | - |
| EKI57_RS21630 (EKI57_21630) | guaC | 4214133..4215176 (+) | 1044 | WP_001217338.1 | GMP reductase | - |
| EKI57_RS21635 (EKI57_21635) | hofC | 4215211..4216413 (-) | 1203 | WP_000157234.1 | protein transport protein HofC | - |
| EKI57_RS21640 (EKI57_21640) | pilB | 4216403..4217788 (-) | 1386 | WP_001025155.1 | type II secretion system protein GspE | Machinery gene |
| EKI57_RS21645 (EKI57_21645) | pilA | 4217798..4218238 (-) | 441 | WP_000360902.1 | prepilin peptidase-dependent pilin | Machinery gene |
| EKI57_RS21650 (EKI57_21650) | nadC | 4218441..4219334 (-) | 894 | WP_001135174.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| EKI57_RS21655 (EKI57_21655) | ampD | 4219422..4219973 (+) | 552 | WP_000923721.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| EKI57_RS21660 (EKI57_21660) | ampE | 4219970..4220824 (+) | 855 | WP_000172006.1 | beta-lactamase regulator AmpE | - |
| EKI57_RS21665 (EKI57_21665) | aroP | 4220867..4222240 (-) | 1374 | WP_012817732.1 | aromatic amino acid transporter AroP | - |
Sequence
Protein
Download Length: 146 a.a. Molecular weight: 15630.76 Da Isoelectric Point: 4.5559
>NTDB_id=309073 EKI57_RS21645 WP_000360902.1 4217798..4218238(-) (pilA) [Escherichia coli strain CFSAN027343]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN
Nucleotide
Download Length: 441 bp
>NTDB_id=309073 EKI57_RS21645 WP_000360902.1 4217798..4218238(-) (pilA) [Escherichia coli strain CFSAN027343]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Haemophilus influenzae 86-028NP |
41.791 |
91.781 |
0.384 |
| pilA | Haemophilus influenzae Rd KW20 |
41.045 |
91.781 |
0.377 |