Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E1J56_RS01455 Genome accession   NZ_CP037910
Coordinates   313874..315370 (+) Length   498 a.a.
NCBI ID   WP_001753144.1    Uniprot ID   -
Organism   Escherichia coli strain YSP8-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308874..320370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1J56_RS01425 tusA 309121..309366 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  E1J56_RS01430 zntA 309468..311666 (-) 2199 WP_000106547.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  E1J56_RS01435 yhhN 311740..312366 (-) 627 WP_000964718.1 lysoplasmalogenase -
  E1J56_RS01440 yhhM 312507..312866 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  E1J56_RS01445 yhhL 312869..313138 (-) 270 WP_001295207.1 DUF1145 family protein -
  E1J56_RS01450 rsmD 313128..313724 (-) 597 WP_000743202.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  E1J56_RS01455 pilA 313874..315370 (+) 1497 WP_001753144.1 signal recognition particle-docking protein FtsY Machinery gene
  E1J56_RS01460 ftsE 315373..316041 (+) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  E1J56_RS01465 ftsX 316034..317092 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  E1J56_RS01470 rpoH 317337..318191 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  E1J56_RS01475 livJ 318462..319565 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  E1J56_RS01480 panM 319764..320147 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54636.46 Da        Isoelectric Point: 4.1753

>NTDB_id=308649 E1J56_RS01455 WP_001753144.1 313874..315370(+) (pilA) [Escherichia coli strain YSP8-1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEHAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=308649 E1J56_RS01455 WP_001753144.1 313874..315370(+) (pilA) [Escherichia coli strain YSP8-1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCAGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment