Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EZZ25_RS06360 Genome accession   NZ_CP036499
Coordinates   1299896..1300381 (-) Length   161 a.a.
NCBI ID   WP_000649332.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294896..1305381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS06340 (EZZ25_06340) coaE 1295401..1296009 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  EZZ25_RS06345 (EZZ25_06345) pilD 1296006..1296881 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  EZZ25_RS06350 (EZZ25_06350) pilC 1296939..1298165 (-) 1227 WP_000648513.1 type II secretion system F family protein Machinery gene
  EZZ25_RS06355 (EZZ25_06355) pilB 1298208..1299896 (-) 1689 WP_000957192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ25_RS06360 (EZZ25_06360) pilA 1299896..1300381 (-) 486 WP_000649332.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EZZ25_RS06365 (EZZ25_06365) nadC 1300626..1301516 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EZZ25_RS06370 (EZZ25_06370) ampD 1301814..1302359 (+) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EZZ25_RS06375 (EZZ25_06375) fldB 1302510..1303028 (-) 519 WP_000690112.1 flavodoxin FldB -
  EZZ25_RS06380 (EZZ25_06380) xerD 1303166..1304074 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  EZZ25_RS06385 (EZZ25_06385) dsbC 1304091..1304843 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16458.81 Da        Isoelectric Point: 8.8070

>NTDB_id=308097 EZZ25_RS06360 WP_000649332.1 1299896..1300381(-) (pilA) [Vibrio cholerae strain 20000]
MKAYKNKQQQGFTLIELMIVVAIIGILAAFAVPAYQNYTNKTHASELLNASSAIKAGVGVCLLSGYSDCKQGANNVPATQ
TFDKGSSDKFAITSGVEVTSPATSSAAATITGAVVATVDATAGKAGLKKSGTLTLTPKLTTSGVTWTITCDLGSQDYCPK
G

Nucleotide


Download         Length: 486 bp        

>NTDB_id=308097 EZZ25_RS06360 WP_000649332.1 1299896..1300381(-) (pilA) [Vibrio cholerae strain 20000]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGCATTTT
GGCTGCTTTTGCTGTACCAGCATACCAAAACTATACAAATAAAACTCATGCATCAGAATTACTCAATGCATCATCAGCAA
TTAAAGCTGGTGTAGGTGTGTGTCTTTTAAGTGGCTACTCTGATTGCAAACAAGGTGCTAACAATGTCCCAGCTACACAA
ACTTTTGATAAAGGAAGTTCGGATAAGTTTGCAATTACTTCAGGTGTAGAAGTCACAAGCCCTGCAACGTCGAGCGCAGC
AGCAACAATCACTGGAGCTGTCGTTGCGACAGTGGATGCAACTGCTGGCAAAGCTGGTTTGAAGAAATCCGGCACATTAA
CTTTAACACCCAAATTAACCACATCAGGAGTCACGTGGACTATCACATGTGATCTAGGCTCACAAGATTACTGTCCAAAA
GGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

38.235

100

0.404
  pilA Haemophilus influenzae 86-028NP

40.69

90.062

0.366
  pilA2 Legionella pneumophila str. Paris

39.456

91.304

0.36
  comP Acinetobacter baylyi ADP1

37.908

95.031

0.36

Multiple sequence alignment