Detailed information NTG0305869

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EXE31_RS07535
• Genome accession: NZ_CP035855
• Coordinates: 1512999..1514009 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli TW11681
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1507999..1519009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXE31_RS07515	mepM	1508966..1510288 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
EXE31_RS07520	znuA	1510304..1511236 (-)	933	WP_001383098.1	zinc ABC transporter substrate-binding protein ZnuA	-
EXE31_RS07525	znuC	1511315..1512070 (+)	756	WP_000202986.1	zinc ABC transporter ATP-binding protein ZnuC	-
EXE31_RS07530	znuB	1512067..1512852 (+)	786	WP_000571458.1	zinc ABC transporter permease subunit ZnuB	-
EXE31_RS07535	ruvB	1512999..1514009 (-)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EXE31_RS07540	ruvA	1514018..1514629 (-)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
EXE31_RS07545	yobI	1514768..1514833 (-)	66	WP_072094247.1	stress response small protein YobI	-
EXE31_RS07550	yebB	1514904..1515506 (+)	603	WP_001024909.1	YebB family permuted papain-like enzyme	-
EXE31_RS07555	ruvC	1515508..1516029 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
EXE31_RS07560	yebC	1516064..1516804 (-)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
EXE31_RS07565	nudB	1516833..1517285 (-)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=305869 EXE31_RS07535 WP_000568519.1 1512999..1514009(-) (ruvB) [Escherichia coli TW11681]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=305869 EXE31_RS07535 WP_000568519.1 1512999..1514009(-) (ruvB) [Escherichia coli TW11681]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494