Detailed information NTG0030573

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFASM23_RS06100
• Genome accession: NC_010577
• Coordinates: 1354813..1355943 (+)
• Length: 376 a.a.
• NCBI ID: WP_012382642.1
• Uniprot ID: -
• Organism: Xylella fastidiosa M23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1349813..1360943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFASM23_RS06075 (XfasM23_1217)	-	1350100..1350924 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
XFASM23_RS06085 (XfasM23_1219)	-	1351558..1352385 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
XFASM23_RS06090 (XfasM23_1220)	-	1352743..1352955 (-)	213	WP_004087301.1	hypothetical protein	-
XFASM23_RS06095 (XfasM23_1221)	pilT	1353613..1354647 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFASM23_RS06100 (XfasM23_1222)	pilU	1354813..1355943 (+)	1131	WP_012382642.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFASM23_RS06105 (XfasM23_1223)	-	1356636..1357604 (-)	969	WP_004087304.1	YafY family protein	-
XFASM23_RS06110 (XfasM23_1224)	-	1358453..1359262 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
XFASM23_RS06115 (XfasM23_1225)	hemC	1359316..1360233 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42460.68 Da
• Isoelectric Point: 6.5023

>NTDB_id=30573 XFASM23_RS06100 WP_012382642.1 1354813..1355943(+) (pilU) [Xylella fastidiosa M23]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DHIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=30573 XFASM23_RS06100 WP_012382642.1 1354813..1355943(+) (pilU) [Xylella fastidiosa M23]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCACATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399