Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EXA05_RS07855 Genome accession   NZ_CP035826
Coordinates   1609180..1610661 (+) Length   493 a.a.
NCBI ID   WP_001219900.1    Uniprot ID   -
Organism   Escherichia coli strain 67-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1604180..1615661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA05_RS07835 gspB 1604349..1604768 (-) 420 WP_000461450.1 putative general secretion pathway protein GspB -
  EXA05_RS07840 gspA 1604770..1606239 (-) 1470 WP_000107576.1 type II secretion system protein GspA -
  EXA05_RS07845 gspC 1606419..1607234 (+) 816 WP_001142708.1 type II secretion system protein GspC -
  EXA05_RS07850 gspD 1607218..1609170 (+) 1953 WP_001326512.1 type II secretion system secretin GspD -
  EXA05_RS07855 pilB 1609180..1610661 (+) 1482 WP_001219900.1 type II secretion system ATPase GspE Machinery gene
  EXA05_RS07860 gspF 1610658..1611854 (+) 1197 WP_001109570.1 type II secretion system inner membrane protein GspF -
  EXA05_RS07865 gspG 1611864..1612301 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  EXA05_RS07870 gspH 1612309..1612818 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  EXA05_RS07875 gspI 1612815..1613192 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  EXA05_RS07880 gspJ 1613185..1613772 (+) 588 WP_000610633.1 type II secretion system minor pseudopilin GspJ -
  EXA05_RS07885 gspK 1613765..1614748 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54626.52 Da        Isoelectric Point: 6.1000

>NTDB_id=305381 EXA05_RS07855 WP_001219900.1 1609180..1610661(+) (pilB) [Escherichia coli strain 67-1]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQITVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=305381 EXA05_RS07855 WP_001219900.1 1609180..1610661(+) (pilB) [Escherichia coli strain 67-1]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGTCAGTGCCAGACCATGACCAGTTTGT
CACAGGCTGATTTCGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCTTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTACGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40.966

96.552

0.396
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383

Multiple sequence alignment