Detailed information NTG0304242

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PLA107_RS30920
• Genome accession: NZ_CP031225
• Coordinates: 5445404..5446468 (-)
• Length: 354 a.a.
• NCBI ID: WP_002551652.1
• Uniprot ID: A0A7Z6Y2Y4
• Organism: Pseudomonas amygdali pv. lachrymans str. M301315
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5440404..5451468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PLA107_RS30895 (PLA107_026470)	aroK	5440985..5441503 (-)	519	WP_002551657.1	shikimate kinase AroK	-
PLA107_RS30900 (PLA107_026475)	pilQ	5441508..5443613 (-)	2106	WP_005743117.1	type IV pilus secretin PilQ	Machinery gene
PLA107_RS30905 (PLA107_026480)	pilP	5443661..5444188 (-)	528	WP_002551655.1	type 4a pilus biogenesis lipoprotein PilP	-
PLA107_RS30910 (PLA107_026485)	pilO	5444185..5444808 (-)	624	WP_002551654.1	type 4a pilus biogenesis protein PilO	-
PLA107_RS30915 (PLA107_026490)	-	5444805..5445404 (-)	600	WP_004654254.1	PilN domain-containing protein	-
PLA107_RS30920 (PLA107_026495)	pilM	5445404..5446468 (-)	1065	WP_002551652.1	pilus assembly protein PilM	Machinery gene
PLA107_RS30925 (PLA107_026500)	-	5446675..5449119 (+)	2445	WP_005743118.1	penicillin-binding protein 1A	-
PLA107_RS30945 (PLA107_026520)	-	5449863..5451131 (-)	1269	WP_002551650.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38078.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=304242 PLA107_RS30920 WP_002551652.1 5445404..5446468(-) (pilM) [Pseudomonas amygdali pv. lachrymans str. M301315]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=304242 PLA107_RS30920 WP_002551652.1 5445404..5446468(-) (pilM) [Pseudomonas amygdali pv. lachrymans str. M301315]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGATGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GAATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGCCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGATTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTCTTTGCAGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCTAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7Z6Y2Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469