Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MG068_RS16745 Genome accession   NZ_CP031167
Coordinates   3606692..3607750 (-) Length   352 a.a.
NCBI ID   WP_010484061.1    Uniprot ID   A0A0L8AA94
Organism   Stenotrophomonas sp. ASS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3601692..3612750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG068_RS16725 (MG068_16690) - 3602615..3604588 (-) 1974 WP_049422631.1 type IV pilus secretin PilQ family protein -
  MG068_RS16730 (MG068_16695) - 3604610..3605116 (-) 507 WP_010484064.1 pilus assembly protein PilP -
  MG068_RS16735 (MG068_16700) pilO 3605143..3605799 (-) 657 WP_049400744.1 type 4a pilus biogenesis protein PilO -
  MG068_RS16740 (MG068_16705) - 3605796..3606692 (-) 897 WP_132810709.1 PilN domain-containing protein -
  MG068_RS16745 (MG068_16710) pilM 3606692..3607750 (-) 1059 WP_010484061.1 pilus assembly protein PilM Machinery gene
  MG068_RS16750 (MG068_16715) - 3607970..3610393 (+) 2424 WP_132810710.1 penicillin-binding protein 1A -
  MG068_RS16755 (MG068_16720) - 3610786..3611430 (+) 645 WP_049461747.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37753.41 Da        Isoelectric Point: 4.4089

>NTDB_id=303840 MG068_RS16745 WP_010484061.1 3606692..3607750(-) (pilM) [Stenotrophomonas sp. ASS1]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPAELDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIEGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=303840 MG068_RS16745 WP_010484061.1 3606692..3607750(-) (pilM) [Stenotrophomonas sp. ASS1]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGTGGCAATCGTTTTCGTGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCCATCCGCCGCGCGATGAACCGCTCGGGGAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCTGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTCGACGAGAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTACCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTGGAACTGCGCCAGTCGGCGCTGGAGCTGGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTATGCCCTGGTCGCCAGCGAGCTGCCGGT
ATCCATCGAGGGTGTGGTCGCGCTGGTCGACATCGGTGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAGTTCAACCGCGTTGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTGGGCGGTCTGCCGGAAATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGCTGGCCCAGGATGCCCCCGCGCTGATGATCGCAACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8AA94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.578

99.716

0.474

Multiple sequence alignment