Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   DN409_RS26265 Genome accession   NZ_CP031071
Coordinates   5041606..5042955 (-) Length   449 a.a.
NCBI ID   WP_149191148.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN401     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5036606..5047955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS26240 (DN409_26250) - 5038153..5039085 (-) 933 WP_149191056.1 YafY family protein -
  DN409_RS26245 (DN409_26255) - 5039168..5039611 (+) 444 WP_149191057.1 VOC family protein -
  DN409_RS26250 (DN409_26260) hpf 5039715..5040257 (-) 543 WP_002034758.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DN409_RS26255 (DN409_26265) cspC 5040578..5040775 (-) 198 WP_001990088.1 cold shock protein CspC -
  DN409_RS26260 (DN409_26270) - 5040902..5041606 (-) 705 WP_002129929.1 ComF family protein -
  DN409_RS26265 (DN409_26275) comFA 5041606..5042955 (-) 1350 WP_149191148.1 ATP-dependent helicase ComFA Machinery gene
  DN409_RS26270 (DN409_26280) - 5043086..5044417 (-) 1332 WP_061689029.1 NlpC/P60 family protein -
  DN409_RS26275 (DN409_26285) - 5044682..5044996 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  DN409_RS26280 (DN409_26290) - 5045170..5046012 (-) 843 WP_002016143.1 DegV family protein -
  DN409_RS26285 (DN409_26295) - 5046251..5046886 (+) 636 WP_002034752.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50869.29 Da        Isoelectric Point: 9.6091

>NTDB_id=303232 DN409_RS26265 WP_149191148.1 5041606..5042955(-) (comFA) [Bacillus mycoides strain BPN401]
MLAGRQLLLKELSSDLQDKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFASLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=303232 DN409_RS26265 WP_149191148.1 5041606..5042955(-) (comFA) [Bacillus mycoides strain BPN401]
ATGTTAGCTGGTAGACAGTTACTGTTAAAAGAACTTTCTTCAGATTTACAGGATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTACAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGCATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATTCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTGAAAAATTTACAAGTCGCAGTTTTAGGGGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATTTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.567

98.218

0.497
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365

Multiple sequence alignment