Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   DUN60_RS12980 Genome accession   NZ_CP031055
Coordinates   2905472..2906101 (-) Length   209 a.a.
NCBI ID   WP_004729667.1    Uniprot ID   -
Organism   Vibrio splendidus strain Vibrio sp     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2900472..2911101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS12960 aroB 2901405..2902493 (-) 1089 WP_114634066.1 3-dehydroquinate synthase -
  DUN60_RS12965 aroK 2902518..2903036 (-) 519 WP_004729664.1 shikimate kinase AroK -
  DUN60_RS12970 pilQ 2903213..2904931 (-) 1719 WP_054542267.1 type IV pilus secretin PilQ family protein Machinery gene
  DUN60_RS12975 pilP 2904967..2905485 (-) 519 WP_114634067.1 pilus assembly protein PilP Machinery gene
  DUN60_RS12980 pilO 2905472..2906101 (-) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  DUN60_RS12985 pilN 2906094..2906693 (-) 600 WP_114634068.1 PilN domain-containing protein Machinery gene
  DUN60_RS12990 pilM 2906677..2907702 (-) 1026 WP_114634069.1 type IV pilus assembly protein PilM Machinery gene
  DUN60_RS12995 - 2907854..2910415 (+) 2562 WP_167409363.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24294.86 Da        Isoelectric Point: 4.6751

>NTDB_id=302976 DUN60_RS12980 WP_004729667.1 2905472..2906101(-) (pilO) [Vibrio splendidus strain Vibrio sp]
MANLQNKISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=302976 DUN60_RS12980 WP_004729667.1 2905472..2906101(-) (pilO) [Vibrio splendidus strain Vibrio sp]
ATGGCTAACCTGCAAAATAAGATTAGTTGGCAAAACAGGATGAGTTTGCAAGATCTCGATGTTGATGAGATTACCGAATG
GCCATTGCTGCCTCAGCTTCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGC
CGCTCGACGATGAACTGCAACAGATGAAGCAACAAGAGCAGACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCGGCCTTGCCTAAGCTCCAGAGCCAGCTGGATGAGCTAACTAGCCGTTATGACTATCTATTGGAGCAATTGCCCGTTCA
AAAAGAGCTAGCCAGTATGTTGGCCTCTGTGAACGAGCTTGGATTGGATAATAAGCTGACATTTACGCGAATTGACTGGG
GGCAGAAACAGAACAAAGAGTTTCTCTATCGTTTACCGCTCAACATCGAGCTGACGGGTGACTACCACGAGATTGGTGAT
TTCTCTGCGGCCATTGCTAAGCTGCCGCGCATCATCAGCTTTGATGACGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTCAGGGTTCGAGCTTATACCTACCAATTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.166

92.344

0.703
  pilO Vibrio campbellii strain DS40M4

73.514

88.517

0.651

Multiple sequence alignment