Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DUN60_RS12990 Genome accession   NZ_CP031055
Coordinates   2906677..2907702 (-) Length   341 a.a.
NCBI ID   WP_114634069.1    Uniprot ID   -
Organism   Vibrio splendidus strain Vibrio sp     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2901677..2912702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUN60_RS12965 aroK 2902518..2903036 (-) 519 WP_004729664.1 shikimate kinase AroK -
  DUN60_RS12970 pilQ 2903213..2904931 (-) 1719 WP_054542267.1 type IV pilus secretin PilQ family protein Machinery gene
  DUN60_RS12975 pilP 2904967..2905485 (-) 519 WP_114634067.1 pilus assembly protein PilP Machinery gene
  DUN60_RS12980 pilO 2905472..2906101 (-) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  DUN60_RS12985 pilN 2906094..2906693 (-) 600 WP_114634068.1 PilN domain-containing protein Machinery gene
  DUN60_RS12990 pilM 2906677..2907702 (-) 1026 WP_114634069.1 type IV pilus assembly protein PilM Machinery gene
  DUN60_RS12995 - 2907854..2910415 (+) 2562 WP_167409363.1 PBP1A family penicillin-binding protein -
  DUN60_RS13000 oxyR 2910528..2911421 (-) 894 WP_114634071.1 DNA-binding transcriptional regulator OxyR -
  DUN60_RS24755 - 2911420..2911620 (+) 201 WP_114634072.1 hypothetical protein -
  DUN60_RS13010 - 2911643..2912371 (+) 729 WP_004729672.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38059.61 Da        Isoelectric Point: 7.2077

>NTDB_id=302978 DUN60_RS12990 WP_114634069.1 2906677..2907702(-) (pilM) [Vibrio splendidus strain Vibrio sp]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQMASRLYPEKKNWLLVDVGIDQTSLGIIPQGLAPFYKDIAFGTKDLRSNGAASDIESVFATTEDTH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQMSCESLNPLSLFENKVAKRRRLPTNRQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=302978 DUN60_RS12990 WP_114634069.1 2906677..2907702(-) (pilM) [Vibrio splendidus strain Vibrio sp]
ATGGGTTCATCATTAATTACAGGCATAGATATAAATCATCACAGCATCAAAGCTGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGTCTGCCTTTTGGCTGTTGCAACGTCGCCATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCGATAGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTACCAGGTATACGCCACACGTAAAGAAGTGGTGGATAGCCGAGCGGATGCTTTGACCAAAGCTGGTTTTAAACCT
GTGGTGGTAGACACGCAGGCGCATGGACTGCTTAACATCTGGCAAATGGCATCGCGCCTATACCCCGAAAAGAAAAACTG
GCTGCTGGTGGATGTGGGAATCGACCAAACGTCGCTAGGCATTATTCCACAAGGTTTGGCTCCGTTTTATAAAGATATCG
CTTTTGGTACTAAAGATCTTCGCAGCAATGGTGCTGCAAGTGATATCGAGAGTGTGTTCGCCACCACTGAAGATACACAT
CAATTCATTGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTCTATTCCTCGGGAAATGCGCAGCAACCTATCTC
CGGGATCTGGCTGATGGGCGAGGGCGCCAGCATTCCGATGGTAACCGAAGAGCTAGAACGTCATTTTCAGATGAGTTGCG
AGTCTCTGAATCCTTTGTCTCTATTTGAGAATAAGGTGGCGAAGCGACGTCGATTACCAACGAATCGGCAACATTTCGGT
ATTGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.824

99.707

0.537
  pilM Vibrio campbellii strain DS40M4

47.076

100

0.472

Multiple sequence alignment