Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DPR03_RS10605 Genome accession   NZ_CP030754
Coordinates   2213415..2214536 (+) Length   373 a.a.
NCBI ID   WP_048881346.1    Uniprot ID   A0A1P8PHY9
Organism   Acinetobacter schindleri strain H3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2208415..2219536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPR03_RS10590 - 2210381..2211487 (+) 1107 WP_071319901.1 acyltransferase family protein -
  DPR03_RS10595 - 2211535..2212221 (-) 687 WP_071319900.1 YggS family pyridoxal phosphate-dependent enzyme -
  DPR03_RS10600 pilT 2212345..2213382 (+) 1038 WP_004811122.1 type IV pilus twitching motility protein PilT Machinery gene
  DPR03_RS10605 pilU 2213415..2214536 (+) 1122 WP_048881346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DPR03_RS10610 fur 2214613..2215050 (-) 438 WP_004811125.1 ferric iron uptake transcriptional regulator -
  DPR03_RS10615 - 2215163..2215561 (+) 399 WP_004811126.1 outer membrane protein assembly factor BamE -
  DPR03_RS10620 - 2215671..2215997 (-) 327 WP_004811128.1 RnfH family protein -
  DPR03_RS10625 - 2215997..2217076 (-) 1080 WP_071319899.1 hypothetical protein -
  DPR03_RS10630 - 2217216..2217671 (-) 456 WP_004893331.1 bacteriohemerythrin -
  DPR03_RS10635 - 2217947..2218795 (-) 849 WP_071319898.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41451.65 Da        Isoelectric Point: 6.8885

>NTDB_id=301333 DPR03_RS10605 WP_048881346.1 2213415..2214536(+) (pilU) [Acinetobacter schindleri strain H3]
MDFNGLLDYMVAQKASDLFITADVEPSIKVNGSIVPIGSVKLPGPAVGQILHSIMTEKQRKEFADTRECNFAITNPSKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDDLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAMVAQQLIPTIDGTGRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGAHLMNAGSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=301333 DPR03_RS10605 WP_048881346.1 2213415..2214536(+) (pilU) [Acinetobacter schindleri strain H3]
ATGGATTTTAACGGCTTACTCGACTATATGGTGGCGCAAAAGGCATCGGATCTGTTCATTACTGCAGATGTCGAACCTTC
AATTAAAGTGAATGGCTCAATTGTACCGATTGGATCAGTCAAATTACCTGGACCAGCAGTAGGCCAGATTCTGCATTCAA
TTATGACGGAAAAACAGCGCAAAGAATTTGCCGATACACGTGAGTGTAACTTTGCGATTACCAATCCCAGCAAATCAGCA
CGTTTCCGTGTCAGTGCATTTCAGCAGCGTGATCAGCCGGGTATGGTTCTGCGTCGTATTGAAACCGTCATTCCTACTAT
GGATGATTTAAAGTTACCGCCAATTCTGAAAGAACTGGCGATGACCAAACGCGGTATTATCATCTTTGTGGGCGCAACGG
GTACCGGTAAATCGACTTCCCTCGCCTCTTTGATTGGTTATCGTAATGAAAATTCCAAAGGTCATATCATTACCATCGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGGTGTATCATCACCCAGCGTGAAGTCGGGATCGACACAGATTCATTTGA
AATCGCCCTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCCCGTGAAACCATGGACT
ATGCCATTGCCTTTGCGGAAACCGGTCACCTGGTATTTGCCACCCTGCACGCCAATAACGCCAACCAGGCCATTGACCGG
ATTATTCACTTCTTTGAAGCAGACCGTCATAACCAGCTGTTTATGGACTTGTCTTTAAATCTCAAAGCGATGGTGGCACA
GCAGCTAATCCCAACCATTGATGGCACAGGCCGTCGTGCCGCAATTGAGATTCTGATCAACTCACCTTTAGTTTCAGATT
TGATCCGTAAAGGTGATGTGCATGAAATTAAAGACTTGATGAAGCGTTCACGTGAACTTGGAATGCAGACTTTTGACCAG
GCACTGTATGACCTGTACAAGGCCAAACAGATCAGCTACAAGGATGCGCTGAAACATGCCGACTCTCCAAACGATCTGCG
TCTGCAAATCAAGCTATCCGAAGAAGGTGGTGCACATCTGATGAATGCCGGCAGCAATATTACCTTTGATGGGCAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8PHY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.545

100

0.745
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

57.265

94.102

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.322

97.319

0.402
  pilT Legionella pneumophila strain Lp02

43.75

90.08

0.394
  pilT Legionella pneumophila strain ERS1305867

43.75

90.08

0.394
  pilT Acinetobacter nosocomialis M2

42.274

91.957

0.389
  pilT Acinetobacter baumannii D1279779

42.274

91.957

0.389
  pilT Acinetobacter baumannii strain A118

42.274

91.957

0.389
  pilT Pseudomonas aeruginosa PAK

40.751

92.761

0.378
  pilT Neisseria meningitidis 8013

40.462

92.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.462

92.761

0.375
  pilT Acinetobacter baylyi ADP1

40.233

91.957

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.884

92.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.102

0.367
  pilT Vibrio cholerae strain A1552

39.031

94.102

0.367

Multiple sequence alignment