Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   DRF63_RS01035 Genome accession   NZ_CP030753
Coordinates   222332..223630 (+) Length   432 a.a.
NCBI ID   WP_043991718.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain S4074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217332..228630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRF63_RS01010 (DRF63_01010) - 217504..220071 (-) 2568 WP_005595965.1 penicillin-binding protein 1A -
  DRF63_RS01015 (DRF63_01015) - 220210..220890 (+) 681 WP_005595967.1 hypothetical protein -
  DRF63_RS01020 (DRF63_01020) - 220872..221393 (+) 522 WP_005595970.1 hypothetical protein -
  DRF63_RS01025 (DRF63_01025) - 221492..221923 (+) 432 WP_005595972.1 hypothetical protein -
  DRF63_RS01030 (DRF63_01030) - 221916..222320 (+) 405 WP_005595974.1 hypothetical protein -
  DRF63_RS01035 (DRF63_01035) comE 222332..223630 (+) 1299 WP_043991718.1 type IV pilus secretin PilQ Machinery gene
  DRF63_RS01040 (DRF63_01040) nusB 223756..224169 (+) 414 WP_005595979.1 transcription antitermination factor NusB -
  DRF63_RS01045 (DRF63_01045) thiL 224234..225199 (+) 966 WP_043991719.1 thiamine-phosphate kinase -
  DRF63_RS01050 (DRF63_01050) - 225212..225685 (+) 474 WP_005595983.1 phosphatidylglycerophosphatase A -
  DRF63_RS01055 (DRF63_01055) - 225694..226320 (+) 627 WP_005595985.1 LysE family transporter -
  DRF63_RS01060 (DRF63_01060) - 226379..227416 (-) 1038 WP_005595987.1 TrmH family RNA methyltransferase -
  DRF63_RS01065 (DRF63_01065) suhB 227582..228394 (+) 813 WP_005595989.1 inositol-1-monophosphatase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47748.89 Da        Isoelectric Point: 9.4227

>NTDB_id=301275 DRF63_RS01035 WP_043991718.1 222332..223630(+) (comE) [Actinobacillus pleuropneumoniae strain S4074]
MRKMIWLLLFILTPVMANTFSLSLKNAPTAEILSYLAEEYGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTSAY
EKQIYFIGHKKDEKAATAIGVNSDLLKPKLITKTIKLDYAKAAEVIESLTKGSGHFLSENGYLHFDDRSNSLIIKDSPES
MKNIVQLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGIFAPTNGHHKIAGSLEANGLPNTNRLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEVPYVLYSRKDEVKNIEFKEAVLGLQVTPHISNDK
QILLDLVVTQNSPNSTGSTLQGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDKI
SKRELVIFVTPYIVKNGQPQISSHSSQKLAPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=301275 DRF63_RS01035 WP_043991718.1 222332..223630(+) (comE) [Actinobacillus pleuropneumoniae strain S4074]
ATGCGAAAAATGATTTGGTTGCTCCTGTTTATATTAACGCCGGTTATGGCAAATACTTTTTCTCTCTCGCTGAAAAATGC
GCCGACTGCGGAAATTTTAAGTTATTTAGCCGAAGAATACGGTAAAAATATCGTGTTAAGCGACAATATTGAGACAAATA
CTACATTAAGAATTGAAAATAGTGATTTTGACAGCGTATTAAAAAGTATTACTCGGGCGAATAAACTCACGAGTGCATAC
GAAAAACAGATCTATTTTATCGGGCATAAAAAAGATGAGAAAGCGGCTACGGCAATAGGCGTAAATTCTGATTTATTGAA
ACCTAAATTGATCACTAAGACAATAAAATTAGATTATGCGAAGGCGGCGGAAGTGATTGAATCTTTAACTAAAGGAAGCG
GCCATTTTTTATCGGAAAACGGCTATTTGCATTTTGATGATCGTAGTAACAGTTTGATTATTAAAGACAGTCCGGAATCG
ATGAAAAATATCGTGCAATTAATCAGAAACTTAGATAAACCGACCGAACAGATTGCAATTGAAGCCAGAATTGTCACGAT
AAGCAGTGAGAATTTACAAGAATTAGGTGTGCGTTGGGGGATATTTGCTCCTACAAACGGACACCATAAAATTGCCGGTT
CGCTTGAAGCGAACGGATTACCGAATACTAATCGTTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCACTA
CAAGTGGCGAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATAATGTTGAGATTATCGC
CAGCCCTCGTTTATTAACCACCAATAAGAAGCCGGCAAGCATTAAGCAAGGAACGGAAGTTCCTTATGTGCTGTATAGCC
GTAAAGACGAAGTGAAAAATATCGAATTTAAAGAAGCGGTTTTAGGTTTACAAGTGACGCCGCATATTTCAAACGATAAG
CAAATTTTGCTCGATTTGGTAGTCACCCAAAACTCACCTAATTCGACCGGTTCAACCCTACAAGGCTTAGTGACGATTGA
TAAGCAGGAACTGAATACGCAAGTATTTGCTCAGCATGGCGAAACGATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCGATTCCGGTACTTAAAAAGTTATTTAGCCATTCTAGTGATAAAATT
AGTAAGCGTGAACTAGTGATTTTTGTGACGCCTTACATTGTTAAAAACGGACAACCGCAAATTTCCTCACATTCTTCACA
GAAATTAGCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.747

96.065

0.641
  comE Haemophilus influenzae Rd KW20

52.804

99.074

0.523
  comE Haemophilus influenzae 86-028NP

52.57

99.074

0.521
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.651

96.065

0.41
  pilQ Vibrio cholerae strain A1552

42.651

96.065

0.41
  pilQ Vibrio campbellii strain DS40M4

40.559

99.306

0.403

Multiple sequence alignment