Detailed information NTG0301049

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: ETT70_RS00335
• Genome accession: NZ_CP035429
• Coordinates: 54365..55363 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain emmNA
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49365..60363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETT70_RS00320 (ETT70_00320)	-	50101..51441 (+)	1341	WP_023611329.1	hypothetical protein	-
ETT70_RS00325 (ETT70_00325)	purB	51810..53102 (+)	1293	WP_023612104.1	adenylosuccinate lyase	-
ETT70_RS00330 (ETT70_00330)	comR	53234..54145 (+)	912	WP_014635220.1	XRE family transcriptional regulator	Regulator
ETT70_RS09285	comS	54236..54334 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
ETT70_RS00335 (ETT70_00335)	ruvB	54365..55363 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
ETT70_RS00340 (ETT70_00340)	-	55501..55938 (+)	438	WP_136112731.1	low molecular weight protein-tyrosine-phosphatase	-
ETT70_RS00345 (ETT70_00345)	-	55961..56362 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
ETT70_RS00350 (ETT70_00350)	-	56359..58134 (+)	1776	WP_032460527.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=301049 ETT70_RS00335 WP_002986679.1 54365..55363(+) (ruvB) [Streptococcus pyogenes strain emmNA]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=301049 ETT70_RS00335 WP_002986679.1 54365..55363(+) (ruvB) [Streptococcus pyogenes strain emmNA]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGTTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473