Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CSC44_RS17500 Genome accession   NZ_CP030327
Coordinates   3652000..3653064 (+) Length   354 a.a.
NCBI ID   WP_023103573.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_458     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3647000..3658064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC44_RS17490 (CSC44_3525) - 3647971..3649239 (+) 1269 WP_003103278.1 malic enzyme -
  CSC44_RS17495 (CSC44_3526) - 3649344..3651815 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  CSC44_RS17500 (CSC44_3527) pilM 3652000..3653064 (+) 1065 WP_023103573.1 type IV pilus assembly protein PilM Machinery gene
  CSC44_RS17505 (CSC44_3528) pilN 3653064..3653660 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  CSC44_RS17510 (CSC44_3529) pilO 3653657..3654280 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  CSC44_RS17515 (CSC44_3530) pilP 3654277..3654801 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  CSC44_RS17520 (CSC44_3531) pilQ 3654855..3656987 (+) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  CSC44_RS17525 (CSC44_3532) aroK 3656999..3657517 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37964.43 Da        Isoelectric Point: 4.5399

>NTDB_id=300880 CSC44_RS17500 WP_023103573.1 3652000..3653064(+) (pilM) [Pseudomonas aeruginosa strain AR_458]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPEHVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=300880 CSC44_RS17500 WP_023103573.1 3652000..3653064(+) (pilM) [Pseudomonas aeruginosa strain AR_458]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCACGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACTGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment