Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DPH57_RS23210 Genome accession   NZ_CP030092
Coordinates   5384767..5386227 (+) Length   486 a.a.
NCBI ID   WP_112939840.1    Uniprot ID   A0A7U5Y2V6
Organism   Massilia sp. YMA4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5379767..5391227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPH57_RS23185 (DPH57_23185) - 5381235..5382257 (+) 1023 WP_373888291.1 SIS domain-containing protein -
  DPH57_RS23190 (DPH57_23190) - 5382257..5382796 (+) 540 WP_227470304.1 HAD family hydrolase -
  DPH57_RS23195 (DPH57_23195) lptC 5382809..5383411 (+) 603 WP_112939837.1 LPS export ABC transporter periplasmic protein LptC -
  DPH57_RS23200 (DPH57_23200) lptA 5383421..5383981 (+) 561 WP_112939838.1 lipopolysaccharide transport periplasmic protein LptA -
  DPH57_RS23205 (DPH57_23205) lptB 5383984..5384760 (+) 777 WP_112939839.1 LPS export ABC transporter ATP-binding protein -
  DPH57_RS23210 (DPH57_23210) rpoN/rpoN1 5384767..5386227 (+) 1461 WP_112939840.1 RNA polymerase factor sigma-54 Machinery gene
  DPH57_RS23215 (DPH57_23215) hpf 5386312..5386662 (+) 351 WP_112939841.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DPH57_RS23220 (DPH57_23220) - 5387864..5388943 (+) 1080 WP_112939842.1 fatty acid desaturase -
  DPH57_RS23225 (DPH57_23225) - 5389024..5390028 (+) 1005 WP_112939843.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
  DPH57_RS23230 (DPH57_23230) - 5390060..5390881 (+) 822 WP_112939844.1 hypothetical protein -

Sequence

Protein


Download         Length: 486 a.a.        Molecular weight: 54158.69 Da        Isoelectric Point: 5.1156

>NTDB_id=299072 DPH57_RS23210 WP_112939840.1 5384767..5386227(+) (rpoN/rpoN1) [Massilia sp. YMA4]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSLRLLADGAIGQQSAPEAPAEGPP
QQADAPAPAEAERYDGEGGPEGDSDWSDVGRNKGPDDDDARPQLEASQHTLREHLMEQMRVTVQEPRDRALVELIIDALD
ENGYLEESLEEIHGRLPEELDVEMEELRTALALLQSFDPVGVGARSASECLALQIRKMPGVPLVTRRMALCIVEKHLAWF
AQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASDVSDYVVPDVIVKKGRNGWVVTLNNDVMPRLRVNALYASLL
KQGKGESQMSAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQKYM
LTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQFTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALKIPPV
SLRKSL

Nucleotide


Download         Length: 1461 bp        

>NTDB_id=299072 DPH57_RS23210 WP_112939840.1 5384767..5386227(+) (rpoN/rpoN1) [Massilia sp. YMA4]
ATGAAACAGTCTCTGCAACTGCGAACGTCGCAGCACCTCGCGCTGACCCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTGGAGCTGCATCAGGAACTGGAACAGCTGCTGACCGATAACCCGCTGCTGGAACGCCTGGACGACCCGC
TCGACCGTTCGCTGCGGCTGCTGGCCGATGGTGCCATCGGCCAGCAGAGCGCACCCGAGGCGCCTGCCGAGGGCCCGCCG
CAGCAGGCCGACGCCCCCGCCCCGGCAGAAGCGGAACGGTACGACGGCGAAGGCGGCCCGGAAGGCGACAGCGACTGGAG
CGACGTCGGTCGCAACAAGGGGCCGGACGACGACGACGCCCGCCCGCAGCTGGAGGCGAGCCAGCACACGCTGCGCGAAC
ACCTGATGGAGCAGATGCGCGTGACGGTGCAGGAGCCGCGCGACCGCGCCCTGGTCGAGCTCATCATCGACGCGCTGGAC
GAGAACGGCTACCTGGAGGAAAGCCTGGAAGAGATCCACGGCCGCCTGCCGGAAGAGCTGGACGTGGAAATGGAAGAGCT
GCGCACGGCGTTGGCGCTGCTGCAGAGCTTCGACCCGGTCGGCGTGGGCGCGCGCAGCGCGTCCGAGTGCCTGGCGCTGC
AGATCCGCAAGATGCCGGGCGTGCCGCTGGTGACGCGCCGGATGGCGCTGTGCATCGTCGAGAAGCACCTGGCCTGGTTC
GCCCAGCGCGACTTTAACAAGCTGAAAAAGGCGCTCGACTGCGACGACGAGGACCTGCGCGAAGCGCAGGCCGTGATCCG
CCAGTGCAATCCGCATCCGGGCGCCGCGTTCGCGTCCGACGTGTCAGACTATGTCGTACCGGATGTGATTGTGAAGAAGG
GCCGCAATGGCTGGGTCGTCACGCTGAACAACGACGTGATGCCGCGCCTGCGGGTGAACGCGCTGTATGCCAGCCTGCTG
AAGCAAGGCAAGGGCGAGTCGCAGATGAGCGCGCAACTGCAGGAAGCGAAGTGGCTGATCAAGAACATGCGCCAGCGCTT
CGACACGATCCTTCGCGTCGCCCAGGCGATCGTCGAGCGCCAGAAGAACTTTTTCTCGCACGGCGCCGTGGCGATGAGAC
CCCTTGTGTTGCGTGAAATAGCTGATACACTGGGGCTACACGAGAGCACCATCTCTCGGGTGACAACTCAAAAATATATG
CTGACCCCACACGGCATGTTTGAGTTGAAGTACTTTTTTGGCAGCCACGTCGCAACCGAAGCTGGGGGAGAAGCTTCCTC
GACTGCGATCCGTGCGCTGATCGTGCAATTCACAGGAGCCGAAGACCCGAAGAACCCTTTATCCGACAGTAAGATTGCGG
ACATGCTGGGAGAACAGGGCATGGTGATTGCGCGACGAACTGTTGCCAAGTATCGTGAAGCCCTGAAAATCCCTCCCGTC
AGCCTCCGCAAGTCCTTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(124-316)

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-484)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.516

100

0.628

Multiple sequence alignment