Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   CT138_RS05445 Genome accession   NZ_CP030062
Coordinates   1105208..1106521 (-) Length   437 a.a.
NCBI ID   WP_112110693.1    Uniprot ID   -
Organism   Mannheimia varigena strain Teagasc     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1100208..1111521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CT138_RS05415 (CT138_05415) ruvB 1100833..1101837 (-) 1005 WP_112110687.1 Holliday junction branch migration DNA helicase RuvB -
  CT138_RS05420 (CT138_05420) ruvA 1101848..1102453 (-) 606 WP_112110688.1 Holliday junction branch migration protein RuvA -
  CT138_RS05425 (CT138_05425) - 1102561..1103187 (-) 627 WP_112110689.1 LysE family transporter -
  CT138_RS05430 (CT138_05430) - 1103193..1103666 (-) 474 WP_112110690.1 phosphatidylglycerophosphatase A -
  CT138_RS05435 (CT138_05435) thiL 1103672..1104652 (-) 981 WP_112110691.1 thiamine-phosphate kinase -
  CT138_RS05440 (CT138_05440) nusB 1104715..1105128 (-) 414 WP_112110692.1 transcription antitermination factor NusB -
  CT138_RS05445 (CT138_05445) comE 1105208..1106521 (-) 1314 WP_112110693.1 type IV pilus secretin PilQ Machinery gene
  CT138_RS05450 (CT138_05450) - 1106530..1106943 (-) 414 WP_112110694.1 hypothetical protein -
  CT138_RS05455 (CT138_05455) - 1106945..1107478 (-) 534 WP_112110695.1 hypothetical protein -
  CT138_RS05460 (CT138_05460) - 1107480..1108001 (-) 522 WP_112110696.1 hypothetical protein -
  CT138_RS05465 (CT138_05465) - 1108081..1108677 (-) 597 WP_239493887.1 competence protein ComA -
  CT138_RS05470 (CT138_05470) - 1108803..1111355 (+) 2553 WP_112110697.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48405.57 Da        Isoelectric Point: 9.2324

>NTDB_id=298826 CT138_RS05445 WP_112110693.1 1105208..1106521(-) (comE) [Mannheimia varigena strain Teagasc]
MFRIFLYLLLSSSLALADTVSLSVKNAPTSLLLTYLSEETGKNIVLEDNIQTKSILRLENKSIEEIFKTISKVNKLSFSQ
ESDIVYISKKEEKLADLNSAPIAHLQNNIQNPPLVTSPKLITKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKKLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGADHYKFGGRLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNTQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVRGMKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=298826 CT138_RS05445 WP_112110693.1 1105208..1106521(-) (comE) [Mannheimia varigena strain Teagasc]
ATGTTTAGAATTTTTCTTTATTTATTACTTAGTTCCTCATTAGCATTGGCTGATACTGTTTCTCTTTCAGTAAAAAATGC
ACCAACTTCACTTCTACTTACTTATTTATCTGAAGAAACAGGAAAAAATATAGTGTTAGAGGATAATATTCAAACTAAAT
CCATCTTAAGATTAGAAAATAAGTCTATTGAGGAGATATTCAAAACGATCAGTAAAGTAAATAAATTATCTTTTAGCCAA
GAAAGTGATATTGTTTATATCAGTAAAAAAGAAGAAAAATTGGCTGATTTAAATTCAGCTCCAATAGCCCATTTGCAAAA
TAATATACAAAACCCACCGCTTGTAACCTCTCCAAAACTGATAACTAAAACCATAAAACTAAATTATGCGAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGTAGTGGTACGTTTTTATCTGAGAATGGCTATATCCATTTTGACGAGCGAAGTAAT
AGCTTAATTATTAAAGATAGTGCGAAATCACTGCAAAATATTGAGCGATTAGTCAAAAAACTAGACCAACCTACAGAACA
AATTGCGATTGAAGCTCGCATCGTTACTATCAGTAGCGAACATTTGCAAGAACTGGGGGTGCGTTGGGGAATGTTTTCCA
GAGGAGCTGATCACTATAAATTTGGCGGACGACTTGAAGGTAATGGATTAAGTAATGTAGCTAATAATCTCAATGTGAAT
TTCCCTGTTGCGAATGGAGCTTCCGCCGTTTTACAAGTGGCTTCCATTAATAGCCGTGTGCTAGATTTGGAACTTAGTGC
ATTAGAGCAGGAAAACAGTGTGGAAATTATTGCCAGTCCTCGTCTTTTAACCACGAATAAAAAACCTGCAAGCATCAAGC
AAGGCACAGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGTTAGATTTAATCGTCACTCAAAACTCACCGAATACACA
ATCTGGTAGCAGTGGTTTAATTACCATTGATAAACAAGAACTTAATACACAGGTGTTTGCTAAACACGGCGAAACTATCG
TGCTAGGTGGTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAGTACCTATTTTAGGCTCAATTCCGTTTATTAAA
CGATTATTTAGCCAAACAAGAGACAAAATCAGCAAGCGGGAACTTGTTATTTTTGTTACCCCTTACATTCTCCAATCAAA
CGAAAAAGTAAGGGGCATGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.759

99.542

0.625
  comE Haemophilus influenzae Rd KW20

51.09

94.508

0.483
  comE Haemophilus influenzae 86-028NP

50.363

94.508

0.476
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.573

96.568

0.382
  pilQ Vibrio cholerae strain A1552

39.573

96.568

0.382
  pilQ Vibrio campbellii strain DS40M4

37.762

98.169

0.371

Multiple sequence alignment