Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DK875_RS10210 Genome accession   NZ_CP030020
Coordinates   2071441..2072733 (+) Length   430 a.a.
NCBI ID   WP_053866470.1    Uniprot ID   -
Organism   Streptococcus suis strain ISU2514     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 2069107..2077201 2071441..2072733 within 0


Gene organization within MGE regions


Location: 2069107..2077201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK875_RS10200 (DK875_10615) - 2069107..2070309 (-) 1203 WP_002935596.1 IS110 family transposase -
  DK875_RS10205 (DK875_10620) - 2070752..2071384 (-) 633 WP_004194123.1 YigZ family protein -
  DK875_RS10210 (DK875_10625) comFA/cflA 2071441..2072733 (+) 1293 WP_053866470.1 DEAD/DEAH box helicase Machinery gene
  DK875_RS10215 (DK875_10630) comFC/cflB 2072726..2073391 (+) 666 WP_004194119.1 ComF family protein Machinery gene
  DK875_RS10220 (DK875_10635) hpf 2073468..2074010 (+) 543 WP_004194117.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DK875_RS10225 (DK875_10640) - 2074380..2075582 (+) 1203 WP_002935596.1 IS110 family transposase -
  DK875_RS10230 (DK875_10650) - 2075912..2077201 (-) 1290 WP_002935134.1 IS4-like element ISSsu2 family transposase -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48916.53 Da        Isoelectric Point: 9.3663

>NTDB_id=298394 DK875_RS10210 WP_053866470.1 2071441..2072733(+) (comFA/cflA) [Streptococcus suis strain ISU2514]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFKEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQAADNAVKEAGVQVFLTATSTDEL
YKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=298394 DK875_RS10210 WP_053866470.1 2071441..2072733(+) (comFA/cflA) [Streptococcus suis strain ISU2514]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTAAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCAAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
TATAAAAAAGTCAGAACAGGTAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTCCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGAAGGAGTATAGAACGTCCGACTGGT
TTACTTCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

66.821

100

0.67
  comFA/cflA Streptococcus pneumoniae Rx1

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae D39

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae R6

66.125

100

0.663
  comFA/cflA Streptococcus pneumoniae TIGR4

66.125

100

0.663
  comFA/cflA Streptococcus mitis SK321

65.661

100

0.658
  comFA Lactococcus lactis subsp. cremoris KW2

53.904

92.326

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363

Multiple sequence alignment