Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   DK875_RS10015 Genome accession   NZ_CP030020
Coordinates   2039317..2039751 (-) Length   144 a.a.
NCBI ID   WP_002936547.1    Uniprot ID   A0A0Z8H0U1
Organism   Streptococcus suis strain ISU2514     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034317..2044751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK875_RS09990 (DK875_10395) - 2034506..2035762 (-) 1257 WP_002936539.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  DK875_RS09995 (DK875_10400) - 2035816..2036367 (-) 552 WP_014636756.1 folate family ECF transporter S component -
  DK875_RS10000 (DK875_10410) - 2036682..2037869 (-) 1188 WP_002936543.1 acetate kinase -
  DK875_RS10005 (DK875_10415) comYH 2037919..2038872 (-) 954 WP_002936545.1 class I SAM-dependent methyltransferase Machinery gene
  DK875_RS10010 (DK875_10420) comGG 2038929..2039339 (-) 411 WP_002936546.1 competence type IV pilus minor pilin ComGG -
  DK875_RS10015 (DK875_10425) comGF/cglF 2039317..2039751 (-) 435 WP_002936547.1 competence type IV pilus minor pilin ComGF Machinery gene
  DK875_RS10020 (DK875_10430) comYE 2039738..2040031 (-) 294 WP_002936548.1 competence type IV pilus minor pilin ComGE Machinery gene
  DK875_RS10025 (DK875_10435) comGD 2040115..2040423 (-) 309 WP_002936549.1 competence type IV pilus minor pilin ComGD -
  DK875_RS10030 (DK875_10440) comYC 2040404..2040685 (-) 282 WP_002936550.1 competence type IV pilus major pilin ComGC Machinery gene
  DK875_RS10035 (DK875_10445) comYB 2040687..2041559 (-) 873 WP_053866392.1 type II secretion system F family protein Machinery gene
  DK875_RS10040 (DK875_10450) comYA 2041471..2042421 (-) 951 WP_002936562.1 competence type IV pilus ATPase ComGA Machinery gene
  DK875_RS10045 (DK875_10455) - 2042507..2043457 (+) 951 WP_002936565.1 S66 peptidase family protein -
  DK875_RS10050 (DK875_10460) - 2043492..2043857 (-) 366 WP_002936568.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16580.00 Da        Isoelectric Point: 6.9819

>NTDB_id=298386 DK875_RS10015 WP_002936547.1 2039317..2039751(-) (comGF/cglF) [Streptococcus suis strain ISU2514]
MLKSKIPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRLEWDQSDLVKVENGKVYVNKVE
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAIYQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=298386 DK875_RS10015 WP_002936547.1 2039317..2039751(-) (comGF/cglF) [Streptococcus suis strain ISU2514]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTTT
CTAGCCAGTTGCGGTTGGAGTGGGACCAGTCGGACTTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGTCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTACCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATTTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z8H0U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae D39

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae R6

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae TIGR4

54.61

97.917

0.535
  comGF/cglF Streptococcus mitis NCTC 12261

55.224

93.056

0.514
  comGF/cglF Streptococcus mitis SK321

52.239

93.056

0.486
  comYF Streptococcus mutans UA140

48.611

100

0.486
  comYF Streptococcus mutans UA159

47.917

100

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

45.39

97.917

0.444

Multiple sequence alignment