Detailed information NTG0029686

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SUB_RS06220
• Genome accession: NC_012004
• Coordinates: 1229901..1232159 (-)
• Length: 752 a.a.
• NCBI ID: WP_012658719.1
• Uniprot ID: B9DUQ7
• Organism: Streptococcus uberis 0140J
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1224901..1237159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SUB_RS06195 (SUB1239)	nagB	1224954..1225658 (-)	705	WP_012658714.1	glucosamine-6-phosphate deaminase	-
SUB_RS06200 (SUB1240)	queA	1225839..1226867 (+)	1029	WP_012658715.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
SUB_RS06205 (SUB1241)	-	1226907..1227947 (-)	1041	WP_041817641.1	serine hydrolase	-
SUB_RS06210 (SUB1242)	sodA	1228113..1228721 (-)	609	WP_012658717.1	superoxide dismutase SodA	-
SUB_RS06215 (SUB1243)	holA	1228818..1229858 (-)	1041	WP_012658718.1	DNA polymerase III subunit delta	-
SUB_RS06220 (SUB1244)	comEC/celB	1229901..1232159 (-)	2259	WP_012658719.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SUB_RS06225 (SUB1245)	comEA/celA/cilE	1232140..1232820 (-)	681	WP_012658720.1	helix-hairpin-helix domain-containing protein	Machinery gene
SUB_RS06230 (SUB1246)	-	1232883..1233623 (-)	741	WP_012658721.1	lysophospholipid acyltransferase family protein	-
SUB_RS06235 (SUB1247)	-	1233746..1234531 (+)	786	WP_046388252.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
SUB_RS06240 (SUB1248)	-	1234572..1234841 (+)	270	WP_012658723.1	GIY-YIG nuclease family protein	-
SUB_RS06245 (SUB1249)	-	1234863..1236860 (-)	1998	WP_041817644.1	KUP/HAK/KT family potassium transporter	-

Sequence

Protein

• Length: 752 a.a.
• Molecular weight: 86062.41 Da
• Isoelectric Point: 10.0562

>NTDB_id=29686 SUB_RS06220 WP_012658719.1 1229901..1232159(-) (comEC/celB) [Streptococcus uberis 0140J]
MTSLLTKFFPIPIPQFSLLCLLVFFAVHFPNKISVCLFLSALVVAFKHRALKRNVKLSCLLMLFASYVYFTKSYQECQFR
QEPTRIESVILVPDSVVINGDSLSFKARKGKNHFQIFYQLKNKKEQDFFKKNNQLLQISGSIELKKAERKRYFNGFDYQD
HLKNQGIYRIGRLKEIKSIGVRKAESFFDYLAIWRRYAIVRSQVQFPKPMSDYMTGLLFGYLDKSFSEMTEIYSQLGIIH
LFALSGMQVSFFLNLFRKGLILSWIPRDFYPILELCFSIIYAGLTGYSISVLRSLLQRNLANMGLKGSTNLCLAFLLMFL
ISPFFPLTIGGVLSFVYAFLLTLIPSENTSGLKGKILGLFYLQLATIPFLILFFSTFHPVSIILTLIFSFLFDILILPLL
TFIFLISPVLTGYHLNSLFQMLEKIMVNVVELFPHPIIFGQPPILVFLSLIFIVALIMAFANRKTYLLSFLIIYFFLLKT
ISIPFENEISIVDVGQGDSILLRDKTNKTLLIDVGGTVAFQGKEDWQKRSSNANAERTLIPYLKSRGIKEIDQLLLTHID
TDHVGDLEVVAKSFKIKEILVSEGSLTNSNFVHRLSKLGNPVTVLKVGNSIDIMNGKLYLIYPWKKGDGRNNDSLVLYGK
LLNKSFLFTGDLEKEGEDAILKRYPSMKTDYLKVGHHGSKGSSSNDFLKVLKPKVAFISAGKDNRFHHPHKETLDRLIEN
HVEILRTDQEGTLRLTGTHQWRLERTHPNLDK

Nucleotide

• Length: 2259 bp

>NTDB_id=29686 SUB_RS06220 WP_012658719.1 1229901..1232159(-) (comEC/celB) [Streptococcus uberis 0140J]
ATGACATCATTATTGACTAAGTTTTTCCCAATTCCCATTCCACAGTTTTCTTTGCTGTGCTTATTGGTCTTTTTTGCTGT
ACATTTCCCAAACAAAATCAGTGTTTGCTTATTCTTATCAGCTTTGGTTGTCGCCTTCAAACATAGGGCATTAAAAAGAA
ATGTAAAGCTTTCTTGTCTTCTTATGTTATTTGCCTCATATGTTTATTTCACCAAATCTTATCAGGAGTGTCAATTCAGG
CAAGAACCAACACGAATCGAATCTGTTATCTTAGTTCCAGATTCTGTTGTTATAAACGGTGATAGTCTATCTTTTAAAGC
AAGAAAAGGAAAAAATCATTTTCAAATTTTTTACCAATTGAAGAACAAAAAAGAGCAAGACTTTTTTAAGAAAAATAATC
AGCTGTTACAAATCAGTGGAAGTATTGAACTCAAAAAAGCTGAGAGGAAACGCTACTTTAATGGTTTTGACTATCAGGAC
CATCTGAAAAATCAAGGAATCTATCGAATTGGTCGTTTGAAAGAGATAAAATCAATTGGCGTTCGAAAAGCTGAAAGTTT
TTTCGATTATTTAGCTATTTGGCGTCGATATGCCATAGTAAGGAGTCAAGTTCAGTTTCCTAAGCCCATGTCTGATTACA
TGACTGGATTATTATTTGGGTATTTGGATAAATCATTCTCTGAGATGACAGAAATTTATAGTCAATTAGGAATCATACAT
CTTTTTGCCTTGTCAGGAATGCAGGTATCCTTCTTTTTAAATCTCTTCCGAAAGGGATTAATCTTAAGTTGGATTCCAAG
AGACTTTTATCCTATTTTAGAATTATGTTTTTCGATTATTTATGCTGGTTTAACAGGTTATAGCATTTCAGTATTAAGAA
GTCTATTGCAGCGAAATTTGGCAAATATGGGTTTGAAAGGAAGTACAAATTTATGCCTGGCTTTTCTCCTAATGTTCTTG
ATATCTCCTTTTTTTCCATTAACCATTGGCGGAGTCTTGTCCTTTGTCTATGCCTTCCTTTTAACTCTCATTCCCTCTGA
AAATACATCAGGATTAAAAGGGAAAATTTTAGGGCTTTTTTACTTACAATTGGCCACTATTCCTTTTTTAATCTTATTTT
TTTCAACATTTCACCCAGTATCAATCATTTTAACACTTATCTTTTCTTTTTTATTCGATATCCTTATTCTTCCCTTGCTT
ACTTTTATTTTTCTAATAAGTCCAGTATTGACCGGTTACCACCTTAACAGTTTATTTCAAATGTTAGAAAAAATCATGGT
CAATGTAGTTGAATTGTTTCCTCATCCGATTATTTTTGGTCAACCACCTATTTTAGTCTTTCTCTCGCTTATCTTTATAG
TTGCATTGATAATGGCTTTTGCTAATCGAAAAACCTATCTCCTGTCATTTTTGATAATTTATTTTTTCCTTTTAAAAACG
ATTTCAATTCCCTTTGAAAATGAGATATCAATTGTGGATGTTGGTCAAGGAGATAGCATTCTTCTGAGAGATAAAACTAA
TAAAACGCTCTTAATTGATGTTGGTGGCACAGTAGCTTTTCAAGGAAAAGAAGATTGGCAAAAAAGAAGCAGCAATGCAA
ATGCAGAAAGAACCTTAATTCCATATTTAAAATCAAGAGGCATTAAAGAAATTGATCAATTACTCTTAACACACATTGAT
ACTGACCATGTTGGAGATTTGGAAGTGGTGGCAAAGTCATTTAAAATAAAAGAGATTCTTGTTAGTGAAGGAAGTTTAAC
AAATAGTAATTTCGTTCATCGTTTGTCAAAATTAGGGAATCCCGTCACCGTCTTAAAAGTTGGTAATAGTATTGATATTA
TGAATGGAAAGCTATACCTCATTTATCCATGGAAAAAGGGAGATGGTCGAAATAATGACTCACTTGTTTTATATGGAAAA
CTCTTAAACAAGTCCTTCCTTTTTACTGGTGATTTAGAAAAGGAAGGAGAAGATGCAATACTAAAAAGATACCCTTCAAT
GAAGACTGATTATTTAAAAGTTGGACATCATGGTTCTAAAGGGTCATCTAGTAACGATTTTCTAAAAGTTCTCAAACCTA
AAGTGGCATTTATTTCGGCGGGAAAAGACAATAGGTTTCATCATCCTCATAAAGAGACCTTAGACAGGCTTATAGAGAAT
CATGTTGAAATACTAAGGACAGATCAAGAGGGGACTCTACGCCTCACAGGGACTCATCAGTGGCGCTTGGAGAGAACGCA
TCCGAATTTAGATAAATAG

Domains

Predicted by InterProScan.

• Competence protein (Competence) (217-461)

• Metallo-beta-lactamase superfamily (Lactamase_B) (493-701)

3D structure

• AlphaFold DB: B9DUQ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	43.742	98.803	0.432
comEC/celB	Streptococcus mitis NCTC 12261	43.607	98.803	0.431
comEC	Lactococcus lactis subsp. cremoris KW2	42.61	99.867	0.426
comEC/celB	Streptococcus pneumoniae TIGR4	42.857	98.67	0.423
comEC/celB	Streptococcus pneumoniae Rx1	42.722	98.67	0.422
comEC/celB	Streptococcus pneumoniae D39	42.722	98.67	0.422
comEC/celB	Streptococcus pneumoniae R6	42.722	98.67	0.422