Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   DKY63_RS15725 Genome accession   NZ_CP029693
Coordinates   3378300..3379631 (+) Length   443 a.a.
NCBI ID   WP_110964938.1    Uniprot ID   A0A2Z4RKX7
Organism   Pseudomonas putida strain JBC17     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3373300..3384631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY63_RS15700 (DKY63_15700) pgeF 3373488..3374213 (-) 726 WP_110964933.1 peptidoglycan editing factor PgeF -
  DKY63_RS15705 (DKY63_15705) rluD 3374210..3375172 (-) 963 WP_110964934.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  DKY63_RS15710 (DKY63_15710) - 3375324..3376346 (+) 1023 WP_110964935.1 outer membrane protein assembly factor BamD -
  DKY63_RS15715 (DKY63_15715) - 3376485..3376718 (+) 234 WP_110964936.1 PP0621 family protein -
  DKY63_RS15720 (DKY63_15720) pilS 3376708..3378297 (+) 1590 WP_110964937.1 ATP-binding protein Regulator
  DKY63_RS15725 (DKY63_15725) pilR 3378300..3379631 (+) 1332 WP_110964938.1 sigma-54 dependent transcriptional regulator Regulator
  DKY63_RS15730 (DKY63_15730) thiO 3379733..3380833 (-) 1101 WP_110964939.1 glycine oxidase ThiO -
  DKY63_RS15735 (DKY63_15735) - 3380892..3381293 (-) 402 WP_110964940.1 type IV pilin protein -
  DKY63_RS15740 (DKY63_15740) - 3381308..3384390 (-) 3083 Protein_3024 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 49437.93 Da        Isoelectric Point: 6.9058

>NTDB_id=295376 DKY63_RS15725 WP_110964938.1 3378300..3379631(+) (pilR) [Pseudomonas putida strain JBC17]
MSPRQKVLVVDDEPDIRELLEITLGRMKLDTYSARNASEALALLKREAFDLCLTDMRLPDGTGLELVQHIQQRYPNLPVA
MITAYGNVDTAINALKAGAFDFLTKPVDLTRLRELVTSALLLPAPGIAIDRRLLGDSLPMRNLRKQIDKLARSQAPVYIS
GESGSGKELVARLIHDRGPRANQPFVPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVADLPLP
MQVKLLRAIQEKAVRSVGGQQEVVVDVRILCATHKDLAAEITAERFRQDLYYRLNVIELRVPPLRERRDDIEPLANHVLK
RLAADTGQPVAKLHPQALDALKSYRFPGNVRELENMLERAHTLCENKQIEAGDLRLAEGNCAADAGVPDLTQIDNLEAYL
ESVERQLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=295376 DKY63_RS15725 WP_110964938.1 3378300..3379631(+) (pilR) [Pseudomonas putida strain JBC17]
ATGAGCCCACGGCAAAAAGTCCTGGTCGTCGATGACGAGCCGGACATCCGCGAACTCCTGGAAATCACCCTGGGACGGAT
GAAACTCGATACCTACAGTGCCCGAAACGCCAGCGAGGCCCTCGCCTTGCTCAAGCGCGAGGCATTCGACCTGTGCCTGA
CCGACATGCGCCTGCCGGACGGCACAGGCCTGGAGCTGGTACAACATATCCAGCAACGCTACCCCAACCTGCCCGTGGCG
ATGATCACCGCCTATGGCAATGTCGACACCGCGATCAACGCACTCAAGGCCGGTGCGTTCGACTTCCTGACCAAGCCGGT
GGACCTCACTCGACTCCGGGAACTGGTCACCAGCGCCCTGCTCCTGCCTGCTCCGGGCATTGCCATCGATCGCCGCTTGC
TGGGCGACTCCCTGCCCATGCGCAACCTTCGCAAGCAAATCGACAAACTGGCGCGCAGCCAGGCGCCGGTGTACATCAGC
GGAGAATCCGGTAGTGGCAAGGAACTGGTCGCCCGCTTGATCCATGACCGCGGACCGAGGGCCAACCAGCCTTTCGTTCC
GGTCAATTGCGGCGCCATCCCGTCGGAACTGATGGAGAGCGAGTTTTTCGGCCATCGCAAAGGCAGCTTCAGCGGCGCTA
TCGAAGACAAGCCCGGACTGTTCCAGGCCGCCCATGGCGGTACCCTGTTTCTCGATGAGGTGGCTGACTTGCCACTGCCC
ATGCAGGTCAAATTGCTGCGGGCGATTCAGGAGAAAGCCGTACGCAGTGTCGGCGGGCAACAGGAAGTGGTTGTCGATGT
ACGTATCCTTTGCGCCACCCACAAAGACCTCGCCGCCGAGATTACCGCCGAGCGCTTCCGCCAGGATTTGTACTATCGCC
TGAACGTGATCGAACTGCGGGTGCCGCCCCTGCGCGAGCGCCGCGACGATATCGAACCGCTGGCAAACCATGTGCTCAAG
CGCCTGGCTGCAGACACCGGCCAACCCGTGGCAAAACTTCACCCGCAGGCCCTCGATGCGCTGAAAAGCTATCGCTTTCC
GGGCAATGTCCGGGAGCTGGAGAACATGCTCGAACGGGCGCATACGCTGTGCGAGAACAAACAGATCGAAGCCGGCGACC
TGCGCCTGGCCGAAGGGAATTGCGCGGCAGATGCCGGGGTGCCGGACCTGACGCAGATCGATAATCTGGAAGCGTACCTG
GAAAGCGTCGAACGCCAGTTGATTCTCCAGGCGCTGGAGGAAACCCGCTGGAACCGCACGGCGGCGGCTCAGCGGTTGAA
TCTTTCATTTCGCTCGATGCGCTACAGGCTGAAGAAACTGGGTTTGGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-116)

Bacterial regulatory protein, Fis family (HTH_8)

(400-440)

Sigma-54 interaction domain (Sigma54_activat)

(134-299)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z4RKX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

78.33

100

0.783
  pilR Acinetobacter baumannii strain A118

49.565

100

0.515
  luxO Vibrio cholerae strain A1552

36.117

100

0.361

Multiple sequence alignment