Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   DL800_RS19815 Genome accession   NZ_CP029692
Coordinates   3335125..3335637 (+) Length   170 a.a.
NCBI ID   WP_000532424.1    Uniprot ID   -
Organism   Escherichia coli strain SD134209     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3301513..3360661 3335125..3335637 within 0
IScluster/Tn 3333718..3334592 3335125..3335637 flank 533


Gene organization within MGE regions


Location: 3301513..3360661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DL800_RS19590 (DL800_19590) - 3301513..3301782 (-) 270 WP_001023452.1 phage tail fiber C-terminal domain-containing protein -
  DL800_RS19595 (DL800_19595) - 3301784..3303106 (-) 1323 WP_000279058.1 prophage tail fiber N-terminal domain-containing protein -
  DL800_RS19600 (DL800_19600) - 3303171..3303770 (-) 600 WP_001230455.1 Ail/Lom family outer membrane beta-barrel protein -
  DL800_RS19605 (DL800_19605) - 3303838..3307314 (-) 3477 WP_000514851.1 host specificity protein J -
  DL800_RS19610 (DL800_19610) - 3307561..3308142 (-) 582 WP_001089947.1 tail assembly protein -
  DL800_RS19615 (DL800_19615) - 3308139..3308882 (-) 744 WP_001369128.1 C40 family peptidase -
  DL800_RS19620 (DL800_19620) - 3308888..3309586 (-) 699 WP_001152113.1 phage minor tail protein L -
  DL800_RS19625 (DL800_19625) - 3309586..3309915 (-) 330 WP_000847298.1 phage tail protein -
  DL800_RS19630 (DL800_19630) - 3309912..3312557 (-) 2646 WP_000918243.1 phage tail tape measure protein -
  DL800_RS19635 (DL800_19635) - 3312607..3312909 (-) 303 Protein_3338 phage tail assembly protein T -
  DL800_RS19640 (DL800_19640) - 3312936..3313358 (-) 423 WP_000479043.1 phage minor tail protein G -
  DL800_RS19645 (DL800_19645) - 3313372..3314124 (-) 753 WP_000235090.1 Ig domain-containing protein -
  DL800_RS19650 (DL800_19650) gpU 3314132..3314530 (-) 399 WP_000682716.1 phage tail terminator protein -
  DL800_RS19655 (DL800_19655) - 3314543..3315166 (-) 624 WP_000974966.1 phage tail protein -
  DL800_RS19660 (DL800_19660) - 3315169..3315450 (-) 282 WP_001281350.1 DNA breaking-rejoining protein -
  DL800_RS19665 (DL800_19665) - 3315443..3315769 (-) 327 WP_001097065.1 capsid cement protein -
  DL800_RS19670 (DL800_19670) - 3315857..3317881 (-) 2025 WP_001114424.1 ClpP-like prohead protease/major capsid protein fusion protein -
  DL800_RS19675 (DL800_19675) - 3317826..3319328 (-) 1503 WP_000974564.1 phage portal protein -
  DL800_RS19680 (DL800_19680) - 3319328..3319540 (-) 213 WP_000102413.1 hypothetical protein -
  DL800_RS19685 (DL800_19685) - 3319537..3321660 (-) 2124 WP_001077625.1 phage terminase large subunit family protein -
  DL800_RS19690 (DL800_19690) - 3321657..3322133 (-) 477 WP_000373407.1 DUF1441 family protein -
  DL800_RS30680 - 3322608..3322793 (-) 186 WP_012816791.1 Rz1 family lipoprotein -
  DL800_RS19700 (DL800_19700) - 3323312..3323392 (-) 81 Protein_3351 lysozyme -
  DL800_RS19705 (DL800_19705) stxB1a 3323797..3324066 (-) 270 WP_000752026.1 Shiga toxin Stx1a subunit B -
  DL800_RS19710 (DL800_19710) stxA1a 3324076..3325023 (-) 948 WP_000691354.1 Shiga toxin Stx1a subunit A -
  DL800_RS19725 (DL800_19725) - 3325530..3325964 (-) 435 WP_001204849.1 antiterminator Q family protein -
  DL800_RS19730 (DL800_19730) - 3325957..3326151 (-) 195 WP_000144764.1 phage NinH family protein -
  DL800_RS19735 (DL800_19735) - 3326148..3326753 (-) 606 WP_001108006.1 recombination protein NinG -
  DL800_RS19740 (DL800_19740) - 3326753..3327476 (-) 724 Protein_3357 phage antirepressor KilAC domain-containing protein -
  DL800_RS19745 (DL800_19745) - 3327551..3328255 (-) 705 WP_000211413.1 phage antirepressor Ant -
  DL800_RS19755 (DL800_19755) - 3328530..3328712 (-) 183 WP_001254256.1 NinE family protein -
  DL800_RS19760 (DL800_19760) - 3328709..3329236 (-) 528 WP_000153268.1 phage N-6-adenine-methyltransferase -
  DL800_RS19765 (DL800_19765) - 3329233..3329679 (-) 447 WP_001303571.1 recombination protein NinB -
  DL800_RS30690 - 3329636..3329872 (-) 237 WP_001449504.1 Lar family restriction alleviation protein -
  DL800_RS30695 - 3329883..3330098 (-) 216 WP_000103678.1 hypothetical protein -
  DL800_RS19775 (DL800_19775) - 3330231..3330509 (-) 279 WP_001000130.1 hypothetical protein -
  DL800_RS19780 (DL800_19780) - 3330579..3330848 (-) 270 WP_001036037.1 hypothetical protein -
  DL800_RS19785 (DL800_19785) - 3330848..3332284 (-) 1437 WP_000131492.1 DnaB-like helicase C-terminal domain-containing protein -
  DL800_RS19790 (DL800_19790) - 3332274..3333173 (-) 900 WP_000065668.1 hypothetical protein -
  DL800_RS19795 (DL800_19795) - 3333166..3333312 (-) 147 WP_000166207.1 DUF2740 family protein -
  DL800_RS19800 (DL800_19800) - 3333347..3333625 (-) 279 WP_001177653.1 lambda phage CII family protein -
  DL800_RS19805 (DL800_19805) - 3333718..3334544 (+) 827 Protein_3370 IS3-like element IS1203 family transposase -
  DL800_RS19810 (DL800_19810) - 3334549..3335124 (+) 576 Protein_3371 Rad52/Rad22 family DNA repair protein -
  DL800_RS19815 (DL800_19815) ssb 3335125..3335637 (+) 513 WP_000532424.1 single-stranded DNA-binding protein Machinery gene
  DL800_RS19820 (DL800_19820) - 3335651..3335944 (+) 294 WP_001111331.1 phage anti-RecBCD protein -
  DL800_RS19825 (DL800_19825) - 3335955..3336122 (+) 168 WP_001214463.1 DUF2737 family protein -
  DL800_RS19830 (DL800_19830) - 3336119..3336553 (+) 435 Protein_3375 ead/Ea22-like family protein -
  DL800_RS30990 - 3336720..3336898 (+) 179 Protein_3376 hypothetical protein -
  DL800_RS19835 (DL800_19835) - 3337031..3337999 (+) 969 Protein_3377 tyrosine-type recombinase/integrase -
  DL800_RS19840 (DL800_19840) - 3338038..3338454 (+) 417 WP_000448925.1 hypothetical protein -
  DL800_RS19845 (DL800_19845) - 3338527..3340275 (-) 1749 WP_000214990.1 response regulator receiver domain -
  DL800_RS19850 (DL800_19850) - 3340277..3341995 (-) 1719 WP_001426837.1 ATP-binding protein -
  DL800_RS19855 (DL800_19855) - 3342120..3343220 (+) 1101 Protein_3381 alpha amylase C-terminal domain-containing protein -
  DL800_RS19860 (DL800_19860) glaH 3343556..3344533 (+) 978 WP_000993087.1 glutarate dioxygenase GlaH -
  DL800_RS19865 (DL800_19865) lhgO 3344553..3345821 (+) 1269 WP_000271938.1 L-2-hydroxyglutarate oxidase -
  DL800_RS19870 (DL800_19870) gabD 3345844..3347292 (+) 1449 WP_000772858.1 NADP-dependent succinate-semialdehyde dehydrogenase -
  DL800_RS19875 (DL800_19875) gabT 3347306..3348586 (+) 1281 WP_001087606.1 4-aminobutyrate--2-oxoglutarate transaminase -
  DL800_RS19880 (DL800_19880) gabP 3348897..3350297 (+) 1401 WP_001344737.1 GABA permease -
  DL800_RS19885 (DL800_19885) csiR 3350318..3350980 (+) 663 WP_000156820.1 DNA-binding transcriptional regulator CsiR -
  DL800_RS19890 (DL800_19890) kbp 3350981..3351430 (-) 450 WP_000522424.1 potassium binding protein Kbp -
  DL800_RS19895 (DL800_19895) yqaE 3351514..3351672 (-) 159 WP_000508177.1 YqaE/Pmp3 family membrane protein -
  DL800_RS19900 (DL800_19900) ygaV 3351855..3352154 (+) 300 WP_000137280.1 metalloregulator ArsR/SmtB family transcription factor -
  DL800_RS19905 (DL800_19905) ygaP 3352164..3352688 (+) 525 WP_001229468.1 thiosulfate sulfurtransferase YgaP -
  DL800_RS19910 (DL800_19910) stpA 3352735..3353139 (-) 405 WP_000115383.1 DNA-binding protein StpA -
  DL800_RS19915 (DL800_19915) alaE 3353807..3354256 (+) 450 WP_000492649.1 L-alanine exporter AlaE -
  DL800_RS19920 (DL800_19920) ygaC 3354293..3354637 (-) 345 WP_000281320.1 DUF2002 family protein -
  DL800_RS19925 (DL800_19925) ygaM 3354789..3355118 (+) 330 WP_001295174.1 DUF883 domain-containing protein -
  DL800_RS19930 (DL800_19930) nrdH 3355366..3355611 (+) 246 WP_001223219.1 glutaredoxin-like protein NrdH -
  DL800_RS19935 (DL800_19935) nrdI 3355608..3356018 (+) 411 WP_000080947.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  DL800_RS19940 (DL800_19940) nrdE 3355991..3358135 (+) 2145 WP_000246506.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  DL800_RS19945 (DL800_19945) nrdF 3358145..3359104 (+) 960 WP_000777939.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  DL800_RS19950 (DL800_19950) proV 3359459..3360661 (+) 1203 WP_000985494.1 glycine betaine/L-proline ABC transporter ATP-binding protein ProV -

Sequence


Protein


Download         Length: 170 a.a.        Molecular weight: 19066.27 Da        Isoelectric Point: 9.4173

>NTDB_id=295352 DL800_RS19815 WP_000532424.1 3335125..3335637(+) (ssb) [Escherichia coli strain SD134209]
MGRGVNRVIIIGRLGQDPEVRYSPSGTAFANLTVATSEQWRDKKTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLE
GKLRTRKWQDQSGQDRFTTEVIVGVGGAMQMLGGKQGSNEQSSPQRNNGQQQRQQSQQHGNYGEPPMNFDDEIPFAPVTL
PFPRHAIHAI

Nucleotide


Download         Length: 513 bp        

>NTDB_id=295352 DL800_RS19815 WP_000532424.1 3335125..3335637(+) (ssb) [Escherichia coli strain SD134209]
ATGGGACGTGGAGTTAATCGCGTAATCATTATCGGTAGATTAGGCCAAGATCCTGAGGTGCGCTATTCACCATCAGGAAC
GGCATTTGCAAACCTTACAGTTGCTACGTCAGAGCAATGGCGTGATAAGAAAACTGGAGAGCAAAAGGAGCAGACGGAGT
GGCACCGCGTGGTAATGAGCGGAAAACTGGCAGAAATTGCCAGCGAATATCTGCGAAAAGGCTCTGAGGTTTATCTTGAA
GGTAAATTGCGGACAAGAAAATGGCAGGATCAAAGCGGACAGGATAGGTTCACTACAGAAGTTATCGTGGGCGTTGGTGG
AGCCATGCAAATGCTTGGTGGCAAGCAAGGAAGCAATGAACAGTCTTCACCTCAGCGAAATAACGGCCAGCAACAAAGAC
AGCAATCTCAGCAGCATGGGAATTACGGCGAACCACCTATGAACTTCGACGATGAAATCCCCTTTGCACCAGTAACTCTC
CCCTTCCCTCGTCACGCTATTCACGCAATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

59.195

100

0.606

  ssb Glaesserella parasuis strain SC1401

49.171

100

0.524

  ssb Neisseria meningitidis MC58

43.452

98.824

0.429

  ssb Neisseria gonorrhoeae MS11

42.424

97.059

0.412


Multiple sequence alignment