Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   EQI27_RS14225 Genome accession   NZ_CP035163
Coordinates   2715086..2716090 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain SRCM103923     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2710086..2721090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI27_RS14195 (EQI27_14195) yrbG 2711060..2711716 (-) 657 WP_015483509.1 DUF421 domain-containing protein -
  EQI27_RS14200 (EQI27_14200) - 2711914..2712303 (+) 390 WP_031315243.1 TIGR04086 family membrane protein -
  EQI27_RS14205 (EQI27_14205) yajC 2712360..2712626 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  EQI27_RS14210 (EQI27_14210) tgt 2712663..2713808 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  EQI27_RS14215 (EQI27_14215) queA 2713835..2714863 (-) 1029 WP_041850173.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  EQI27_RS14220 (EQI27_14220) yrzS 2714893..2715093 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  EQI27_RS14225 (EQI27_14225) ruvB 2715086..2716090 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EQI27_RS14230 (EQI27_14230) ruvA 2716101..2716706 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  EQI27_RS14235 (EQI27_14235) bofC 2716845..2717357 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  EQI27_RS14240 (EQI27_14240) csbX 2717405..2718712 (-) 1308 WP_015483512.1 MFS transporter -
  EQI27_RS14245 (EQI27_14245) iolG 2718783..2719808 (-) 1026 WP_032726305.1 inositol 2-dehydrogenase -
  EQI27_RS14250 (EQI27_14250) yrzF 2720046..2720693 (+) 648 WP_003246159.1 phosphotransferase -
  EQI27_RS14255 (EQI27_14255) - 2720739..2720861 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=292893 EQI27_RS14225 WP_003229718.1 2715086..2716090(-) (ruvB) [Bacillus subtilis strain SRCM103923]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=292893 EQI27_RS14225 WP_003229718.1 2715086..2716090(-) (ruvB) [Bacillus subtilis strain SRCM103923]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTAGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCATCGGCGCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518

Multiple sequence alignment