Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DKG78_RS12330 Genome accession   NZ_CP029466
Coordinates   2539647..2540717 (-) Length   356 a.a.
NCBI ID   WP_132105381.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain HM618     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2534647..2545717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKG78_RS12280 (DKG78_12475) sipW 2534706..2535290 (-) 585 WP_015240205.1 signal peptidase I SipW -
  DKG78_RS12285 (DKG78_12480) tapA 2535262..2535933 (-) 672 WP_069007150.1 amyloid fiber anchoring/assembly protein TapA -
  DKG78_RS12290 (DKG78_12485) - 2536192..2536521 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  DKG78_RS12295 (DKG78_12490) - 2536562..2536741 (-) 180 WP_003153093.1 YqzE family protein -
  DKG78_RS12300 (DKG78_12495) comGG 2536798..2537175 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  DKG78_RS12305 (DKG78_12500) comGF 2537176..2537676 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  DKG78_RS12310 (DKG78_12505) comGE 2537585..2537899 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  DKG78_RS12315 (DKG78_12510) comGD 2537883..2538320 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  DKG78_RS12320 (DKG78_12515) comGC 2538310..2538618 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  DKG78_RS12325 (DKG78_12520) comGB 2538623..2539660 (-) 1038 WP_059368114.1 competence type IV pilus assembly protein ComGB Machinery gene
  DKG78_RS12330 (DKG78_12525) comGA 2539647..2540717 (-) 1071 WP_132105381.1 competence type IV pilus ATPase ComGA Machinery gene
  DKG78_RS12335 (DKG78_12530) - 2540914..2541864 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  DKG78_RS12340 (DKG78_12535) - 2542010..2543311 (+) 1302 WP_012117986.1 hemolysin family protein -
  DKG78_RS12345 (DKG78_12540) - 2543349..2544179 (-) 831 WP_132105383.1 STAS domain-containing protein -
  DKG78_RS12355 (DKG78_12550) - 2544386..2544766 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DKG78_RS12360 (DKG78_12555) - 2545004..2545249 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40614.96 Da        Isoelectric Point: 9.5862

>NTDB_id=292820 DKG78_RS12330 WP_132105381.1 2539647..2540717(-) (comGA) [Bacillus amyloliquefaciens strain HM618]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAKTAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFDVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=292820 DKG78_RS12330 WP_132105381.1 2539647..2540717(-) (comGA) [Bacillus amyloliquefaciens strain HM618]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCAAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGACGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.218

97.753

0.374
  pilF Thermus thermophilus HB27

37.322

98.596

0.368

Multiple sequence alignment