Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   DKE43_RS12320 Genome accession   NZ_CP029464
Coordinates   2347863..2349242 (-) Length   459 a.a.
NCBI ID   WP_276318041.1    Uniprot ID   -
Organism   Bacillus pumilus strain ZB201701     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2342863..2354242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKE43_RS12285 (DKE43_12335) flgL 2343043..2343954 (-) 912 WP_066031682.1 flagellar hook-associated protein FlgL -
  DKE43_RS12290 (DKE43_12340) flgK 2343964..2345484 (-) 1521 WP_060597394.1 flagellar hook-associated protein FlgK -
  DKE43_RS12295 (DKE43_12345) - 2345500..2345982 (-) 483 WP_034660146.1 flagellar protein FlgN -
  DKE43_RS12300 (DKE43_12350) flgM 2346001..2346264 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  DKE43_RS12305 (DKE43_12355) - 2346344..2346763 (-) 420 WP_060597396.1 TIGR03826 family flagellar region protein -
  DKE43_RS18775 comFC 2346820..2347506 (-) 687 WP_189288297.1 ComF family protein Machinery gene
  DKE43_RS12315 (DKE43_12365) - 2347503..2347796 (-) 294 WP_060597398.1 competence protein ComFB -
  DKE43_RS12320 (DKE43_12370) comFA 2347863..2349242 (-) 1380 WP_276318041.1 DEAD/DEAH box helicase Machinery gene
  DKE43_RS12325 (DKE43_12375) - 2349363..2350205 (-) 843 WP_034660152.1 DegV family protein -
  DKE43_RS12330 (DKE43_12380) degU 2350423..2351112 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  DKE43_RS12335 (DKE43_12385) degS 2351177..2352352 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  DKE43_RS12340 (DKE43_12390) - 2352574..2353242 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52576.76 Da        Isoelectric Point: 10.2540

>NTDB_id=292680 DKE43_RS12320 WP_276318041.1 2347863..2349242(-) (comFA) [Bacillus pumilus strain ZB201701]
MNMDKAMELRQQLQSRHLLTAETRCSQSDLDWLVEKGLVIRTPAIERKANGLTCCRCGVSKKRYFAHTPCDMCQKDCAYC
RSCIMMGKTTECGFLYEWTGPQMEETCHTELTWQGNLSKGQKRASEKLIEAIKNKFDLLIWAVCGAGKTEVLFHGIEYAL
HQGMSVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKLAFDVLIIDEVDAFPYSIDE
RLQFAVLKAMRKKGVRVYLSATPSKKMKRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQK
HITKKRRILLFVPSISTMKKITKVLREHRLNVEGVSADDPDRKQKVQHFRDYKYDVLVTTTILERGVTIPNVQVGVLGAE
STIFTESALVQISGRAGRHPDFFKGDVIFFHFGLTRSMKQAKKHIVKMNDTAAKEFSEK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=292680 DKE43_RS12320 WP_276318041.1 2347863..2349242(-) (comFA) [Bacillus pumilus strain ZB201701]
TTGAACATGGACAAAGCAATGGAACTAAGGCAGCAACTGCAGTCACGCCACCTGCTTACCGCTGAAACAAGATGCTCACA
GTCCGATTTGGATTGGCTGGTGGAGAAAGGGTTAGTCATCCGAACACCTGCTATTGAGAGGAAAGCAAATGGTCTTACTT
GCTGTAGATGTGGTGTATCAAAAAAGCGGTACTTTGCCCACACTCCGTGTGACATGTGTCAAAAAGATTGTGCGTACTGT
AGATCATGCATCATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAACATG
TCATACGGAATTAACATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGGGCGTCAGAAAAATTGATCGAAGCGATAAAAA
ACAAATTTGATCTACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATTGAATATGCCTTA
CATCAAGGAATGAGCGTCTGCATTGCGACACCTAGAACAGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCATT
TCAAGGAATGAAAATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGCTAGTGATCGCCACAACTC
ATCAGTTGATGAGATACAAACTTGCATTTGATGTGCTTATTATAGATGAAGTCGACGCCTTTCCTTATTCAATCGATGAA
CGTCTCCAATTTGCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCCTCTAAGAAAAT
GAAAAGAGATGTATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCGTTACCTGTCCCGT
CCTTTCAATGGATTGGACATTGGAAGAAGAAATTAAAAAAGAACCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAA
CATATCACAAAGAAGAGAAGGATTTTACTTTTTGTTCCTTCTATTTCGACCATGAAGAAGATAACGAAGGTCCTTAGAGA
ACACCGCTTAAATGTGGAGGGGGTGTCTGCTGATGATCCAGACAGGAAACAAAAGGTCCAGCACTTCAGAGATTATAAAT
ACGATGTACTAGTTACAACCACCATTCTAGAAAGAGGCGTTACCATTCCAAATGTTCAAGTGGGGGTACTAGGTGCGGAA
TCTACTATCTTTACAGAGAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGT
TATCTTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGTAAAAATGAATGATACAGCTGCAA
AAGAGTTTTCTGAAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(320-419)

Type III restriction enzyme, res subunit (ResIII)

(116-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

54.084

98.693

0.534

Multiple sequence alignment