Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   DJ572_RS20815 Genome accession   NZ_CP029461
Coordinates   4007309..4007827 (-) Length   172 a.a.
NCBI ID   WP_003219228.1    Uniprot ID   A0A063XE16
Organism   Bacillus subtilis strain QB61     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3960715..4007294 4007309..4007827 flank 15


Gene organization within MGE regions


Location: 3960715..4007827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ572_RS20520 (DJ572_20395) yybO 3961131..3962441 (+) 1311 WP_119900000.1 MFS transporter -
  DJ572_RS20525 (DJ572_20400) - 3962602..3963339 (+) 738 WP_042976959.1 MerR family transcriptional regulator -
  DJ572_RS20530 (DJ572_20405) - 3963377..3964165 (-) 789 WP_119900001.1 hypothetical protein -
  DJ572_RS20535 (DJ572_20410) yybH 3964233..3964622 (-) 390 WP_119900003.1 nuclear transport factor 2 family protein -
  DJ572_RS20540 (DJ572_20415) yybG 3964768..3965607 (+) 840 WP_119900005.1 pentapeptide repeat-containing protein -
  DJ572_RS20545 (DJ572_20420) yybF 3965640..3966854 (-) 1215 WP_119900007.1 MFS transporter -
  DJ572_RS20550 (DJ572_20425) yybE 3967042..3967920 (+) 879 WP_015715067.1 LysR family transcriptional regulator -
  DJ572_RS20555 (DJ572_20430) yybD 3967934..3968377 (+) 444 WP_029726103.1 GNAT family N-acetyltransferase -
  DJ572_RS20560 (DJ572_20435) yybC 3968460..3968939 (+) 480 WP_119900009.1 DUF2798 domain-containing protein -
  DJ572_RS21790 (DJ572_20440) - 3968964..3969097 (-) 134 Protein_3991 LysR family transcriptional regulator -
  DJ572_RS20570 (DJ572_20445) yybB 3969114..3969776 (-) 663 WP_116315643.1 MBL fold metallo-hydrolase -
  DJ572_RS20575 (DJ572_20450) yybA 3969946..3970398 (-) 453 WP_003226875.1 MarR family transcriptional regulator -
  DJ572_RS20580 (DJ572_20455) yyaT 3970520..3970960 (+) 441 WP_038429960.1 GNAT family N-acetyltransferase -
  DJ572_RS20585 (DJ572_20460) yyaS 3970962..3971564 (+) 603 WP_017695807.1 YitT family protein -
  DJ572_RS20590 (DJ572_20465) satA 3971632..3972153 (-) 522 WP_119900011.1 streptothricin N-acetyltransferase SatA -
  DJ572_RS20595 (DJ572_20470) yyaQ 3972562..3972918 (+) 357 WP_029726097.1 MmcQ/YjbR family DNA-binding protein -
  DJ572_RS20600 (DJ572_20475) yyaP 3973078..3973644 (+) 567 WP_029726096.1 dihydrofolate reductase family protein -
  DJ572_RS20605 (DJ572_20480) - 3973903..3974053 (+) 151 Protein_3999 DUF255 domain-containing protein -
  DJ572_RS20610 (DJ572_20485) tet(L) 3974156..3975532 (-) 1377 WP_119900013.1 tetracycline efflux MFS transporter Tet(L) -
  DJ572_RS21850 tetL 3975566..3975628 (-) 63 WP_010886645.1 tetracycline resistance efflux system leader peptide -
  DJ572_RS20620 (DJ572_20495) - 3975881..3976051 (+) 171 Protein_4002 DUF255 domain-containing protein -
  DJ572_RS20625 (DJ572_20500) arsC 3976128..3976547 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  DJ572_RS20630 (DJ572_20505) acr3 3976559..3977599 (-) 1041 WP_119900017.1 arsenite efflux transporter Acr3 -
  DJ572_RS20635 (DJ572_20510) arsK 3977621..3978061 (-) 441 WP_119900019.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  DJ572_RS20640 (DJ572_20515) arsR 3978121..3978438 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -
  DJ572_RS20645 (DJ572_20520) - 3978713..3980221 (+) 1509 WP_119900021.1 recombinase family protein -
  DJ572_RS20650 (DJ572_20525) - 3980321..3981103 (-) 783 WP_119900023.1 hypothetical protein -
  DJ572_RS20655 (DJ572_20530) - 3981205..3981597 (-) 393 WP_205590911.1 cystatin-like fold lipoprotein -
  DJ572_RS20660 (DJ572_20535) - 3981651..3982157 (-) 507 WP_119900027.1 hypothetical protein -
  DJ572_RS20665 (DJ572_20540) cwlT 3982172..3983161 (-) 990 WP_119900029.1 bifunctional lytic transglycosylase/C40 family peptidase -
  DJ572_RS20670 (DJ572_20545) - 3983158..3985605 (-) 2448 WP_119900031.1 CD3337/EF1877 family mobilome membrane protein -
  DJ572_RS20675 (DJ572_20550) yddF 3985609..3985935 (-) 327 WP_019716380.1 YddF family protein -
  DJ572_RS20680 (DJ572_20555) conE 3985953..3988448 (-) 2496 WP_119900033.1 VirB4-like ATPase ConE -
  DJ572_RS20685 (DJ572_20560) conD 3988336..3988860 (-) 525 WP_119900035.1 conjugal transfer protein -
  DJ572_RS20690 (DJ572_20565) - 3988873..3989121 (-) 249 WP_119900037.1 hypothetical protein -
  DJ572_RS20695 (DJ572_20570) conB 3989133..3990197 (-) 1065 WP_119900038.1 conjugal transfer protein -
  DJ572_RS21540 - 3990215..3990373 (-) 159 WP_162920081.1 hypothetical protein -
  DJ572_RS20700 (DJ572_20575) - 3990391..3990669 (-) 279 WP_044430163.1 hypothetical protein -
  DJ572_RS20705 (DJ572_20580) - 3990686..3990952 (-) 267 WP_119900040.1 hypothetical protein -
  DJ572_RS20710 (DJ572_20585) nicK 3991219..3992277 (-) 1059 WP_119900042.1 DNA relaxase NicK -
  DJ572_RS20715 (DJ572_20590) - 3992270..3993721 (-) 1452 WP_119900044.1 DNA translocase FtsK -
  DJ572_RS20720 (DJ572_20595) helP 3993757..3994137 (-) 381 WP_119900046.1 helicase processivity factor HelP -
  DJ572_RS20725 - 3994154..3994300 (-) 147 WP_119900048.1 hypothetical protein -
  DJ572_RS20730 (DJ572_20600) - 3994505..3994765 (-) 261 WP_119900050.1 hypothetical protein -
  DJ572_RS20735 (DJ572_20605) - 3994818..3995078 (-) 261 WP_119900052.1 hypothetical protein -
  DJ572_RS20740 (DJ572_20610) - 3995075..3995254 (-) 180 WP_119900054.1 ICEBs1 excisionase -
  DJ572_RS20745 (DJ572_20615) - 3995551..3995934 (+) 384 WP_019716391.1 helix-turn-helix transcriptional regulator -
  DJ572_RS20750 (DJ572_20620) - 3995931..3996461 (+) 531 WP_019716392.1 ImmA/IrrE family metallo-endopeptidase -
  DJ572_RS20755 (DJ572_20625) - 3996574..3997770 (-) 1197 WP_119900056.1 Rap family tetratricopeptide repeat protein -
  DJ572_RS20760 (DJ572_20630) - 3997989..3998756 (+) 768 WP_119900058.1 hypothetical protein -
  DJ572_RS20765 (DJ572_20635) - 3998773..3999597 (+) 825 WP_119900060.1 hypothetical protein -
  DJ572_RS20775 (DJ572_20645) yyaL 3999602..4001691 (+) 2090 Protein_4033 thioredoxin domain-containing protein -
  DJ572_RS20780 (DJ572_20650) yyaK 4001688..4002587 (-) 900 WP_119900140.1 type II CAAX endopeptidase family protein -
  DJ572_RS20785 (DJ572_20655) yyaJ 4002815..4004170 (+) 1356 WP_119900063.1 MFS transporter -
  DJ572_RS20790 (DJ572_20660) maa 4004204..4004758 (-) 555 WP_033884396.1 sugar O-acetyltransferase -
  DJ572_RS20795 (DJ572_20665) yyaH 4004774..4005154 (-) 381 WP_119900065.1 VOC family protein -
  DJ572_RS20800 (DJ572_20670) ccpB 4005210..4006145 (-) 936 WP_119900067.1 transcriptional regulator CcpB -
  DJ572_RS20805 (DJ572_20675) xth 4006204..4006962 (-) 759 WP_029726087.1 exodeoxyribonuclease III -
  DJ572_RS20810 (DJ572_20680) rpsR 4007026..4007265 (-) 240 WP_003219224.1 30S ribosomal protein S18 -
  DJ572_RS20815 (DJ572_20685) ssbA 4007309..4007827 (-) 519 WP_003219228.1 single-stranded DNA-binding protein SsbA Machinery gene

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 18742.31 Da        Isoelectric Point: 4.7621

>NTDB_id=292611 DJ572_RS20815 WP_003219228.1 4007309..4007827(-) (ssbA) [Bacillus subtilis strain QB61]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=292611 DJ572_RS20815 WP_003219228.1 4007309..4007827(-) (ssbA) [Bacillus subtilis strain QB61]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAACGGTGCGGCTGTTGC
TACGTTTACTCTTGCTGTGAATCGTACATTTACGAACCAGTCCGGAGAACGTGAGGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTCTTGAAAAAAGGAAGCCTTGCAGGCGTAGATGGCCGTTTACAAACAAGA
AACTATGAAAACCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCGAAAAA
CGGCGGCGGTTCTGGTTCAGGTGGATACAACGAAGGAAACAGCGGCGGAGGCCAGTACTTTGGCGGAGGCCAAAATGATA
ATCCATTTGGGGGAAATCAAAACAACCAGAGACGCAATCAGGGGAACAGCTTTAATGATGACCCATTTGCCAACGACGGC
AAACCGATTGACATCTCGGATGATGATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A063XE16

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

100

100

1

  ssb Latilactobacillus sakei subsp. sakei 23K

58.192

100

0.599

  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395

  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378


Multiple sequence alignment