Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DDT09_RS12265 Genome accession   NZ_CP029070
Coordinates   2514514..2515584 (-) Length   356 a.a.
NCBI ID   WP_061862635.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain ALB69     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2509514..2520584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDT09_RS12215 (DDT09_12215) - 2509573..2510157 (-) 585 WP_014418370.1 signal peptidase I -
  DDT09_RS12220 (DDT09_12220) tapA 2510129..2510800 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  DDT09_RS12225 (DDT09_12225) - 2511059..2511388 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  DDT09_RS12230 (DDT09_12230) - 2511429..2511608 (-) 180 WP_003153093.1 YqzE family protein -
  DDT09_RS12235 (DDT09_12235) comGG 2511665..2512042 (-) 378 WP_061862389.1 competence type IV pilus minor pilin ComGG Machinery gene
  DDT09_RS12240 (DDT09_12240) comGF 2512043..2512438 (-) 396 WP_061862390.1 competence type IV pilus minor pilin ComGF -
  DDT09_RS12245 (DDT09_12245) comGE 2512452..2512766 (-) 315 WP_061862391.1 competence type IV pilus minor pilin ComGE Machinery gene
  DDT09_RS12250 (DDT09_12250) comGD 2512750..2513187 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  DDT09_RS12255 (DDT09_12255) comGC 2513177..2513443 (-) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  DDT09_RS12260 (DDT09_12260) comGB 2513490..2514527 (-) 1038 WP_061862392.1 competence type IV pilus assembly protein ComGB Machinery gene
  DDT09_RS12265 (DDT09_12265) comGA 2514514..2515584 (-) 1071 WP_061862635.1 competence type IV pilus ATPase ComGA Machinery gene
  DDT09_RS12270 (DDT09_12270) - 2515777..2516727 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  DDT09_RS12275 (DDT09_12275) - 2516873..2518174 (+) 1302 WP_061862393.1 hemolysin family protein -
  DDT09_RS12280 (DDT09_12280) - 2518212..2519042 (-) 831 WP_021494316.1 STAS domain-containing protein -
  DDT09_RS12290 (DDT09_12290) - 2519249..2519629 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DDT09_RS12295 (DDT09_12295) - 2519866..2520111 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40542.85 Da        Isoelectric Point: 9.3531

>NTDB_id=289968 DDT09_RS12265 WP_061862635.1 2514514..2515584(-) (comGA) [Bacillus amyloliquefaciens strain ALB69]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCIEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=289968 DDT09_RS12265 WP_061862635.1 2514514..2515584(-) (comGA) [Bacillus amyloliquefaciens strain ALB69]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCATAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment