Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BTJ40_RS01310 Genome accession   NZ_CP029064
Coordinates   273167..274243 (+) Length   358 a.a.
NCBI ID   WP_108731427.1    Uniprot ID   -
Organism   Microbulbifer sp. A4B17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268167..279243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ40_RS01300 (BTJ40_01300) - 269815..270294 (+) 480 WP_108731425.1 chemotaxis protein CheW -
  BTJ40_RS01305 (BTJ40_01305) - 270358..273018 (-) 2661 WP_108731426.1 penicillin-binding protein 1A -
  BTJ40_RS01310 (BTJ40_01310) pilM 273167..274243 (+) 1077 WP_108731427.1 pilus assembly protein PilM Machinery gene
  BTJ40_RS01315 (BTJ40_01315) - 274245..274820 (+) 576 WP_108731428.1 PilN domain-containing protein -
  BTJ40_RS01320 (BTJ40_01320) - 274833..275450 (+) 618 WP_108731429.1 type 4a pilus biogenesis protein PilO -
  BTJ40_RS01325 (BTJ40_01325) - 275447..275977 (+) 531 WP_108731430.1 pilus assembly protein PilP -
  BTJ40_RS01330 (BTJ40_01330) pilQ 275977..278154 (+) 2178 WP_108731431.1 type IV pilus secretin PilQ -
  BTJ40_RS01335 (BTJ40_01335) aroK 278154..278675 (+) 522 WP_108735122.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38819.10 Da        Isoelectric Point: 4.3360

>NTDB_id=289786 BTJ40_RS01310 WP_108731427.1 273167..274243(+) (pilM) [Microbulbifer sp. A4B17]
MGIFPFLSKGPKAMLGLDISSTAVKLLELSRNGDKYRVESYAVEPLPPNAVVDKNINDVGATAEAIRKVVRRSKTRLREV
AVAVSGSAVITKTLEMPADLSDDALEAQIAIEADQYIPYPLEEVSLDFEVQGPSEKGEGQMEVLLAACRTENIEQRISAL
GEAELEPGVVDVEAYAIERAYTLLEDQLPPQEQLVVAVIDVGATMSALSVLVDGKTVYTREQLFGGRQLTDEIQRRYGLS
AEEAALAKRQGGSGLPDDYFAEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDHILLGGGVAAMEGIGELISEKLGRPTLV
ANPFQKMSVSSRVNRQALSSEAPSLMIAAGLAMREKDY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=289786 BTJ40_RS01310 WP_108731427.1 273167..274243(+) (pilM) [Microbulbifer sp. A4B17]
ATGGGAATTTTCCCTTTTCTGAGCAAAGGCCCCAAGGCCATGTTGGGACTCGATATTAGCTCTACTGCAGTCAAGTTGCT
GGAGCTAAGTCGCAACGGCGATAAATATCGTGTCGAAAGTTACGCCGTGGAACCTCTCCCCCCCAATGCCGTAGTTGATA
AGAATATCAACGATGTGGGGGCGACGGCTGAGGCTATCCGCAAAGTTGTACGACGCTCCAAGACCCGTCTGCGGGAAGTC
GCAGTTGCAGTGTCTGGTTCGGCTGTGATCACCAAGACTCTCGAAATGCCGGCGGATCTCAGTGATGATGCTTTAGAAGC
CCAGATTGCAATCGAAGCCGATCAGTATATTCCCTATCCGTTGGAAGAGGTCAGCCTGGATTTTGAAGTGCAGGGACCTT
CTGAAAAGGGGGAGGGCCAGATGGAAGTGTTGCTCGCAGCTTGCCGCACCGAAAATATTGAGCAGCGCATCTCTGCGCTG
GGTGAGGCTGAGCTGGAGCCCGGTGTTGTGGATGTTGAAGCGTACGCCATTGAGCGAGCTTACACTCTGCTGGAAGACCA
GCTTCCCCCGCAGGAACAATTAGTCGTTGCCGTTATTGATGTCGGGGCAACAATGAGTGCCCTGTCTGTCCTTGTGGATG
GCAAAACGGTTTATACCCGTGAACAGCTTTTTGGCGGACGTCAGCTTACCGATGAAATACAGCGTCGCTATGGACTTTCA
GCGGAAGAGGCTGCGTTGGCAAAACGCCAGGGGGGCAGCGGTCTGCCGGATGACTATTTTGCTGAAGTGCTGGAACCCTT
CCGTGATGCTGTTGTGCAACAGGTAACCCGCTCCTTGCAGTTTTTTTACTCCTCTACATCATATAGTGACGTAGATCATA
TTCTTCTTGGCGGTGGAGTCGCGGCTATGGAAGGAATTGGGGAGCTGATTAGCGAGAAGCTGGGTCGCCCGACATTAGTT
GCTAATCCATTCCAGAAAATGAGCGTCTCTTCACGGGTGAATCGTCAGGCGCTATCAAGTGAAGCCCCTTCACTAATGAT
TGCCGCTGGCTTGGCGATGAGGGAGAAGGACTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.862

97.486

0.506
  comM Acinetobacter nosocomialis M2

51.862

97.486

0.506
  comM Acinetobacter baylyi ADP1

48.555

96.648

0.469
  pilM Legionella pneumophila strain ERS1305867

44.35

98.883

0.439

Multiple sequence alignment