Detailed information NTG0288940

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: DBX28_RS25330
• Genome accession: NZ_CP028959
• Coordinates: 5299094..5300431 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain IMP66
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5294094..5305431

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DBX28_RS25305 (DBX28_25235)	pgeF	5294237..5294965 (-)	729	WP_003123393.1	peptidoglycan editing factor PgeF	-
DBX28_RS25310 (DBX28_25240)	rluD	5294962..5295924 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
DBX28_RS25315 (DBX28_25245)	-	5296070..5297095 (+)	1026	WP_023092932.1	outer membrane protein assembly factor BamD	-
DBX28_RS25320 (DBX28_25250)	-	5297252..5297497 (+)	246	WP_003102591.1	PP0621 family protein	-
DBX28_RS25325 (DBX28_25255)	pilS	5297487..5299079 (+)	1593	WP_003110428.1	two-component system sensor histidine kinase PilS	Regulator
DBX28_RS25330 (DBX28_25260)	pilR	5299094..5300431 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
DBX28_RS25335 (DBX28_25265)	thiO	5300477..5301571 (-)	1095	WP_023518092.1	glycine oxidase ThiO	-
DBX28_RS25340 (DBX28_25270)	-	5301706..5302215 (+)	510	WP_003161431.1	Tfp pilus assembly protein FimT/FimU	-
DBX28_RS25345 (DBX28_25275)	-	5302321..5302827 (+)	507	WP_003102598.1	Tfp pilus assembly protein FimT/FimU	-
DBX28_RS25350 (DBX28_25280)	pilV	5302818..5303375 (+)	558	WP_003119423.1	type 4a pilus minor pilin PilV	Machinery gene
DBX28_RS25355 (DBX28_25285)	pilW	5303372..5304196 (+)	825	WP_003119422.1	type 4a pilus minor pilin PilW	-
DBX28_RS25360 (DBX28_25290)	pilX	5304193..5304780 (+)	588	WP_003112826.1	type 4a pilus minor pilin PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=288940 DBX28_RS25330 WP_003094694.1 5299094..5300431(+) (pilR) [Pseudomonas aeruginosa strain IMP66]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=288940 DBX28_RS25330 WP_003094694.1 5299094..5300431(+) (pilR) [Pseudomonas aeruginosa strain IMP66]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384