Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   DB278_RS02740 Genome accession   NZ_CP028926
Coordinates   566325..567659 (-) Length   444 a.a.
NCBI ID   WP_108511558.1    Uniprot ID   -
Organism   Pasteurella multocida strain 20N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561325..572659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB278_RS02715 (DB278_02715) secM 562843..563157 (-) 315 WP_108511555.1 secA translation cis-regulator SecM -
  DB278_RS02720 (DB278_02720) - 563250..563555 (+) 306 WP_083003370.1 DUF721 domain-containing protein -
  DB278_RS02725 (DB278_02725) - 563601..564506 (-) 906 WP_108511556.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  DB278_RS02730 (DB278_02730) aroB 564510..565598 (-) 1089 WP_108511557.1 3-dehydroquinate synthase -
  DB278_RS02735 (DB278_02735) aroK 565615..566142 (-) 528 WP_005717623.1 shikimate kinase AroK -
  DB278_RS02740 (DB278_02740) comE 566325..567659 (-) 1335 WP_108511558.1 type IV pilus secretin PilQ Machinery gene
  DB278_RS02745 (DB278_02745) - 567669..568031 (-) 363 WP_108511559.1 pilus assembly protein PilP -
  DB278_RS02750 (DB278_02750) - 568034..568558 (-) 525 WP_108511560.1 ATPase -
  DB278_RS02755 (DB278_02755) - 568558..569073 (-) 516 WP_104884245.1 competence protein ComB -
  DB278_RS02760 (DB278_02760) - 569113..569886 (-) 774 WP_104227539.1 pilus assembly protein PilM -
  DB278_RS02765 (DB278_02765) - 570022..572583 (+) 2562 WP_016534629.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49331.71 Da        Isoelectric Point: 7.1842

>NTDB_id=288604 DB278_RS02740 WP_108511558.1 566325..567659(-) (comE) [Pasteurella multocida strain 20N]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQERKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVQSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIILGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSLPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=288604 DB278_RS02740 WP_108511558.1 566325..567659(-) (comE) [Pasteurella multocida strain 20N]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAAGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACGTCTTCAATAAAATTGCATTTTGCCAAAGCTGCAGAGGTAGTGCAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGAATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGCGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCTGGGAGTTTAGCTGCAAACGGCTTTTCAAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCAGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGATAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCATTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAGGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTTGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.124

100

0.613
  comE Haemophilus influenzae 86-028NP

60.449

100

0.606
  comE Glaesserella parasuis strain SC1401

49.173

95.27

0.468
  pilQ Vibrio campbellii strain DS40M4

39.048

94.595

0.369
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.709

93.018

0.369
  pilQ Vibrio cholerae strain A1552

39.709

93.018

0.369

Multiple sequence alignment