Detailed information NTG0285309

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DA456_RS17470
• Genome accession: NZ_CP028490
• Coordinates: 3998883..3999947 (-)
• Length: 354 a.a.
• NCBI ID: WP_003341394.1
• Uniprot ID: A0A0P9GH14
• Organism: Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3993883..4004947

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA456_RS17445 (DA456_17440)	aroK	3994482..3995000 (-)	519	WP_003341387.1	shikimate kinase AroK	-
DA456_RS17450 (DA456_17445)	pilQ	3995005..3997110 (-)	2106	WP_003422532.1	type IV pilus secretin PilQ	Machinery gene
DA456_RS17455 (DA456_17450)	pilP	3997158..3997685 (-)	528	WP_003316653.1	type 4a pilus biogenesis lipoprotein PilP	-
DA456_RS17460 (DA456_17455)	pilO	3997682..3998305 (-)	624	WP_003316652.1	type 4a pilus biogenesis protein PilO	-
DA456_RS17465 (DA456_17460)	-	3998302..3998883 (-)	582	WP_003316651.1	PilN domain-containing protein	-
DA456_RS17470 (DA456_17465)	pilM	3998883..3999947 (-)	1065	WP_003341394.1	pilus assembly protein PilM	Machinery gene
DA456_RS17475 (DA456_17470)	-	4000154..4002598 (+)	2445	WP_003341396.1	penicillin-binding protein 1A	-
DA456_RS17480 (DA456_17475)	-	4003193..4004461 (-)	1269	WP_003316648.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38134.68 Da
• Isoelectric Point: 4.7090

>NTDB_id=285309 DA456_RS17470 WP_003341394.1 3998883..3999947(-) (pilM) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=285309 DA456_RS17470 WP_003341394.1 3998883..3999947(-) (pilM) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTACTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTTGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGAC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGCGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCCTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCACTGAGCTCCAAGGTCAATGCCGGCGCTCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTAGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0P9GH14

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469