Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   DA456_RS15775 Genome accession   NZ_CP028490
Coordinates   3608976..3610313 (+) Length   445 a.a.
NCBI ID   WP_017279844.1    Uniprot ID   A0AAD0MWD9
Organism   Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3603976..3615313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA456_RS15750 (DA456_15745) pgeF 3604173..3604901 (-) 729 WP_017279845.1 peptidoglycan editing factor PgeF -
  DA456_RS15755 (DA456_15750) rluD 3604898..3605860 (-) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  DA456_RS15760 (DA456_15755) - 3606010..3607032 (+) 1023 WP_003344571.1 outer membrane protein assembly factor BamD -
  DA456_RS15765 (DA456_15760) - 3607152..3607391 (+) 240 WP_032607804.1 PP0621 family protein -
  DA456_RS15770 (DA456_15765) pilS 3607381..3608973 (+) 1593 WP_024692602.1 ATP-binding protein Regulator
  DA456_RS15775 (DA456_15770) pilR 3608976..3610313 (+) 1338 WP_017279844.1 sigma-54 dependent transcriptional regulator Regulator
  DA456_RS15780 (DA456_15775) thiO 3610424..3611527 (-) 1104 WP_029571609.1 glycine oxidase ThiO -
  DA456_RS15785 (DA456_15780) - 3611553..3611960 (-) 408 WP_017279558.1 type IV pilin protein -
  DA456_RS15790 (DA456_15785) - 3611972..3615175 (-) 3204 WP_017279559.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49653.60 Da        Isoelectric Point: 6.2986

>NTDB_id=285305 DA456_RS15775 WP_017279844.1 3608976..3610313(+) (pilR) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPHVPVA
MITAHGNVDTAINALKAGAFDFVTKPVDLGRLRELVNSALSLPGAPPTRSIDNRLLGDSLAMRTLRNQIGKLARSQAPIY
ISGESGSGKELVARLIHEQGPRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAGSV
LKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESVSPQEGDGPSLADIDNLED
YLESVERQLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=285305 DA456_RS15775 WP_017279844.1 3608976..3610313(+) (pilR) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
ATGAGCCAACGGCAAAAAATCCTGATAGTCGATGATGAGCCGGATATTCGCGAACTCCTGGAGATCACGCTGGGACGCAT
GAAACTCGACACCCGCAGTGCACGTAACGTTGCCGAAGCCCATGACTGGCTGGCCCGTGAACCGTTCGACATGTGCCTGA
CCGACATGCGTCTGCCTGATGGTAATGGCCTTGAGCTGGTGCAACACATACAGCACGGCTACCCCCACGTGCCGGTGGCG
ATGATCACTGCACACGGCAATGTGGATACCGCGATCAACGCGCTGAAGGCCGGGGCTTTCGATTTTGTGACCAAGCCGGT
CGACCTCGGCAGGCTGCGCGAGCTGGTCAATAGCGCGCTGAGCCTGCCCGGCGCACCACCCACGCGGAGCATCGACAATC
GACTGCTGGGCGACTCGCTGGCAATGCGTACCTTGCGCAATCAGATCGGCAAGCTGGCCCGCAGCCAGGCGCCGATCTAC
ATCAGCGGCGAGTCGGGCAGCGGCAAGGAGCTTGTAGCGCGTCTTATCCACGAGCAGGGCCCGCGCATCGACAAACCTTT
CATACCGGTGAATTGCGGCGCCATTCCTTCCGAGCTGATGGAGAGCGAATTCTTCGGCCATCGCAAAGGCAGCTTCAGCG
GTGCCCACGAAGACAAGCCCGGTCTGTTTCAAGCGGCGCACACCGGCACGCTGTTTCTCGATGAGGTCGCCGACCTGCCG
CTGGCCATGCAGGTCAAACTGCTGCGCGCCATTCAGGAAAAGTCCATTCGCAGCGTCGGCGGCCAGCAAGAGCAGATCGT
CGACGTGCGCATTCTGTGCGCCACCCACAAGAATCTGAATGCGGAGGTCGCGGCAGGTCGGTTCCGTCAGGATCTGTATT
ACCGATTGAACGTGATCGAAGTGCGCGTGCCCTCATTGCGCGAGCGGCGTGAGGATATCGACCAACTGGCGGGCAGCGTG
CTCAAGCGCCTTGCCGGGAACGGCGCACAACCCGTCGCGCGCCTGAACGCGCAGGCGCTGGAAACGCTGAAGAGCTACCG
CTTCCCCGGTAACGTGCGCGAACTGGAAAACATGCTGGAGCGCGCTTACACGCTGTGCGAGAACGACGAAATCCACGCCA
GCGACCTGCGCCTGACAGAGTCCGTCAGCCCTCAGGAGGGCGACGGCCCGAGCCTGGCCGACATCGACAACCTTGAAGAC
TACCTGGAAAGCGTCGAACGCCAGCTCATTCTGCAGGCGCTTGAAGAAACCCGCTGGAACCGCACCGCTGCAGCAGAAAG
GCTCAGCTTGTCGTTCAGGTCACTGCGCTATCGCCTGAAGAAGCTGGGCCTGGATTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(136-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)

Response regulator receiver domain (Response_reg)

(7-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

77.201

99.551

0.769
  pilR Acinetobacter baumannii strain A118

48.394

100

0.508
  luxO Vibrio cholerae strain A1552

37.613

99.775

0.375

Multiple sequence alignment