Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   DA456_RS15770 Genome accession   NZ_CP028490
Coordinates   3607381..3608973 (+) Length   530 a.a.
NCBI ID   WP_024692602.1    Uniprot ID   A0AAD0IBQ2
Organism   Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3602381..3613973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA456_RS15750 (DA456_15745) pgeF 3604173..3604901 (-) 729 WP_017279845.1 peptidoglycan editing factor PgeF -
  DA456_RS15755 (DA456_15750) rluD 3604898..3605860 (-) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  DA456_RS15760 (DA456_15755) - 3606010..3607032 (+) 1023 WP_003344571.1 outer membrane protein assembly factor BamD -
  DA456_RS15765 (DA456_15760) - 3607152..3607391 (+) 240 WP_032607804.1 PP0621 family protein -
  DA456_RS15770 (DA456_15765) pilS 3607381..3608973 (+) 1593 WP_024692602.1 ATP-binding protein Regulator
  DA456_RS15775 (DA456_15770) pilR 3608976..3610313 (+) 1338 WP_017279844.1 sigma-54 dependent transcriptional regulator Regulator
  DA456_RS15780 (DA456_15775) thiO 3610424..3611527 (-) 1104 WP_029571609.1 glycine oxidase ThiO -
  DA456_RS15785 (DA456_15780) - 3611553..3611960 (-) 408 WP_017279558.1 type IV pilin protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58016.91 Da        Isoelectric Point: 6.8620

>NTDB_id=285304 DA456_RS15770 WP_024692602.1 3607381..3608973(+) (pilS) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQSALHLLSQEQLVGQMID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QSIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNEPRSDLPTLEVLDDGPGVSEQHLSKIFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=285304 DA456_RS15770 WP_024692602.1 3607381..3608973(+) (pilS) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
GTGACCGCTGATTCACCGTCCCTTGTCAGCCCGCAGGCACAGCGGATTCTGCGGCTGTACCACCTTTATCGACTGACCAT
CGGCGTCGTACTGGTGCTGTTGATTTCCAGCAGCCTGGAAACCGAGCTGCTGCAACTGGCCAACCCGAACCTATTCCGCT
CGGGCTGCTGGCTGTATCTGGTGTTCAACATTCTGGTCGTCGTGCTGCTGGAACGCCCCAGCCGTCAGGCACAGTTTTTC
GGCCTGGCGATGACTGACGCGATCATGCTCTCCGCGCTGTTCTATGCAGCGGGCGGGCCTTCAAGCGGTATCGGCAACCT
GTTGATTGCCTCGGTGGCGATCAGCAACGTGTTGCTGCGCGGCCGTATCGGCTTGTTGATCGCTGCGGTTTCGGCCATCG
GCATCATCTACCTGACGTTCTACATCAGCTTCGGCACACCCTCGGTCTCCAACGACTACGTGCAGGCTGGCTCGCTGGGC
GCTTTATGCTTCGCCGCCGCCCTGCTGGTGCAGGCGCTGACAGCGCGCATGCAGGTTAGCGAGGTACTGGCCGAACAGCG
CGCAGCGGATGTCGACAGTCTCGAAGCACTCAACGCACTCATTTTGCAGCGCATGCGTACCGGCATTCTGGTGCTGGATG
CCAATCACCAGGTGCTGCTGGCCAACCAGAGTGCCTTGCACCTGCTGAGCCAGGAGCAACTGGTCGGCCAGATGATCGAC
ACCTGCTCGCCGCAGCTGGTTGAACGCCTGCGCCAGTGGCTCGTCAATCCGATCATGGTGCCGCGCAGTATCACCACCGC
CCCCGTCGGGCTGGTGCTGCAACCGAGCTTTGCCACACTCAACCGTGGCGATCAGCGTCAGACGCTGGTTTTTCTCGAAG
ACCTGTCGCAGATCGCCCACCAGGCGCAGCAGCTCAAGCTCGCCGCACTGGGACGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCCGTCAGTCATGCGGCTCAACTGCTTAATGAATCGGAAGAGCTCAGTGCAGCGGATCGACG
CCTGGGCCAGATCATTCATGAGCAGTCGGGACGCATTGACCGAGTCATCGAAAATGTCCTGCAATTGTCGCGCAGACGCG
AAGCGCAACCCGAACTGCTCGATCTGAAAACCTGGCTGGAGCACTTTGTCGCTGAATTTCGCAGTTCCGCAGCGCCCAAT
CAGTCTATTCATGTCGACATCAGCCCCGGCACGCTGACAACCCGCATGGATTCTGGACAGCTGACGCAAATCATGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGAATCATCCGTCAGCGCAGGTCTGGCTGAAACTGCACAATGAACCGA
GAAGCGATTTGCCTACGCTGGAAGTGCTGGACGATGGGCCGGGGGTCAGCGAGCAACACCTGAGCAAGATCTTCGAACCG
TTTTTCACCACAGAAAGCAAAGGCACTGGCCTTGGTCTATATCTGTCACGTGAACTGTGCGAAAGCAATCAGGCGCATCT
GGAATATGCGCCCCGCCCCACGGGTGGCAGCTGCCTGCGCATTACCTTCGCCCACCCGTTCAAGACGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.017

100

0.611

Multiple sequence alignment