Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DA376_RS01945 Genome accession   NZ_CP028437
Coordinates   357072..357509 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain DR-08     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 352072..362509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA376_RS01910 (DA376_01910) - 352926..354128 (+) 1203 WP_032872800.1 GTP-binding protein -
  DA376_RS01915 (DA376_01915) - 354193..355329 (+) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  DA376_RS01920 (DA376_01920) - 355344..355778 (+) 435 WP_021494203.1 RDD family protein -
  DA376_RS01925 (DA376_01925) - 355851..356174 (+) 324 WP_032872805.1 YckD family protein -
  DA376_RS20135 - 356278..356469 (+) 192 WP_074534339.1 family 1 glycosylhydrolase -
  DA376_RS01935 (DA376_01935) - 356394..356612 (+) 219 Protein_337 family 1 glycosylhydrolase -
  DA376_RS01940 (DA376_01940) nin/comJ 356653..357051 (-) 399 WP_032872809.1 competence protein ComJ Regulator
  DA376_RS01945 (DA376_01945) nucA/comI 357072..357509 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DA376_RS01950 (DA376_01950) hxlB 357866..358423 (-) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  DA376_RS01955 (DA376_01955) hxlA 358420..359055 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  DA376_RS01960 (DA376_01960) - 359287..359649 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=284826 DA376_RS01945 WP_003156588.1 357072..357509(-) (nucA/comI) [Bacillus velezensis strain DR-08]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=284826 DA376_RS01945 WP_003156588.1 357072..357509(-) (nucA/comI) [Bacillus velezensis strain DR-08]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment