Detailed information NTG0284627

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: SM12261_RS01525
• Genome accession: NZ_CP028414
• Coordinates: 289198..290265 (+)
• Length: 355 a.a.
• NCBI ID: WP_000159550.1
• Uniprot ID: F9HAA0
• Organism: Streptococcus mitis NCTC 12261
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 284198..295265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS01510 (SM12261_0296)	amiA3	284712..286700 (+)	1989	WP_000749582.1	peptide ABC transporter substrate-binding protein	Regulator
SM12261_RS01515 (SM12261_0297)	amiC	286767..288263 (+)	1497	WP_000759915.1	ABC transporter permease	Regulator
SM12261_RS01520 (SM12261_0298)	amiD	288263..289189 (+)	927	WP_000103691.1	oligopeptide ABC transporter permease OppC	Regulator
SM12261_RS01525 (SM12261_0299)	amiE	289198..290265 (+)	1068	WP_000159550.1	ABC transporter ATP-binding protein	Regulator
SM12261_RS01530 (SM12261_0300)	amiF	290276..291202 (+)	927	WP_001291284.1	ATP-binding cassette domain-containing protein	Regulator
SM12261_RS01540 (SM12261_0301)	-	291606..292484 (-)	879	WP_001049302.1	LysR family transcriptional regulator	-
SM12261_RS01545 (SM12261_0302)	-	292612..292962 (-)	351	WP_000208711.1	helix-turn-helix domain-containing protein	-
SM12261_RS01550 (SM12261_0303)	-	293236..294861 (+)	1626	WP_000200491.1	malolactic enzyme	-
SM12261_RS01555	-	295063..295182 (+)	120	Protein_254	IS630 family transposase	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 39491.07 Da
• Isoelectric Point: 4.8078

>NTDB_id=284627 SM12261_RS01525 WP_000159550.1 289198..290265(+) (amiE) [Streptococcus mitis NCTC 12261]
MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALVGESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDL
TALSSHKDWEQIRGAKIATIFQDPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFDEYPFQY
SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITHDLGVVASIADKVAVMYAGEIVE
YGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSIPGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAK
TWLLHEDAPKVEKPAVIANLHDKIREKMGFAHLAD

Nucleotide

• Length: 1068 bp

>NTDB_id=284627 SM12261_RS01525 WP_000159550.1 289198..290265(+) (amiE) [Streptococcus mitis NCTC 12261]
ATGACAAAAGAAAAAAATGTAATTTTGACTGCTCGCGATATTGTCGTGGAATTTGACGTTCGTGACAAAGTATTGACAGC
CATTCGCGGCGTTTCCCTTGAACTAGTTGAAGGAGAAGTATTAGCCTTGGTAGGTGAGTCAGGATCAGGGAAATCTGTTT
TGACAAAGACATTCACAGGTATGCTTGAAGAAAACGGTCGCATTGCCCAAGGTAGTATTGACTACCGTGGTCAAGATTTG
ACAGCTTTATCTTCTCACAAGGATTGGGAACAAATTCGTGGCGCTAAGATTGCGACTATCTTCCAAGATCCAATGACTAG
TTTGGACCCAATTAAAACAATTGGTAGTCAGATTACAGAAGTTATTGTAAAACACCAAGGAAAAACAGCTAAAGAAGCGA
AAGAATTGGCCATTGACTACATGAATAAGGTTGGGATTCCAGATGCAGATAGACGTTTTGATGAATACCCATTCCAATAT
TCTGGAGGAATGCGTCAACGTATCGTTATTGCGATTGCTCTTGCCTGCCGACCTGATGTCTTGATTTGTGATGAGCCAAC
GACTGCCTTGGATGTGACCATCCAAGCTCAGATTATTGATTTGTTGAAATCTTTACAAAACGAGTACCATTTCACAACAA
TCTTTATCACCCACGACCTTGGTGTGGTGGCAAGTATTGCAGATAAGGTAGCGGTTATGTATGCAGGAGAAATCGTTGAG
TATGGAACTGTTGAGGAAGTCTTCTATGACCCTCGCCATCCATATACATGGAGTCTCTTGTCTAGCTTGCCTCAGCTTGC
TGATGATAAAGGGGATCTTTACTCAATCCCAGGAACACCTCCGTCACTTTATACTGACCTGAAAGGTGATGCCTTTGCCT
TGCGTTCTGACTATGCAATGCAGATTGACTTCGAACAAAAAGCTCCTCAATTCTCAGTATCAGAGACACATTGGGCTAAA
ACTTGGCTACTTCATGAGGATGCTCCAAAAGTAGAAAAACCAGCTGTGATTGCAAATCTCCATGATAAGATTCGTGAAAA
AATGGGATTTGCCCATCTGGCTGACTAG

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-301)

• ABC transporter (ABC_tran) (29-187)

3D structure

• AlphaFold DB: F9HAA0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	87.887	100	0.879
amiE	Streptococcus thermophilus LMG 18311	86.197	100	0.862
amiE	Streptococcus thermophilus LMD-9	86.197	100	0.862
oppD	Streptococcus mutans UA159	54.261	99.155	0.538