Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   SM12261_RS01530 Genome accession   NZ_CP028414
Coordinates   290276..291202 (+) Length   308 a.a.
NCBI ID   WP_001291284.1    Uniprot ID   V8I8Q2
Organism   Streptococcus mitis NCTC 12261     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 285276..296202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS01515 (SM12261_0297) amiC 286767..288263 (+) 1497 WP_000759915.1 ABC transporter permease Regulator
  SM12261_RS01520 (SM12261_0298) amiD 288263..289189 (+) 927 WP_000103691.1 oligopeptide ABC transporter permease OppC Regulator
  SM12261_RS01525 (SM12261_0299) amiE 289198..290265 (+) 1068 WP_000159550.1 ABC transporter ATP-binding protein Regulator
  SM12261_RS01530 (SM12261_0300) amiF 290276..291202 (+) 927 WP_001291284.1 ATP-binding cassette domain-containing protein Regulator
  SM12261_RS01540 (SM12261_0301) - 291606..292484 (-) 879 WP_001049302.1 LysR family transcriptional regulator -
  SM12261_RS01545 (SM12261_0302) - 292612..292962 (-) 351 WP_000208711.1 helix-turn-helix domain-containing protein -
  SM12261_RS01550 (SM12261_0303) - 293236..294861 (+) 1626 WP_000200491.1 malolactic enzyme -
  SM12261_RS01555 - 295063..295182 (+) 120 Protein_254 IS630 family transposase -

Sequence

Protein


Download         Length: 308 a.a.        Molecular weight: 34777.80 Da        Isoelectric Point: 6.5105

>NTDB_id=284628 SM12261_RS01530 WP_001291284.1 290276..291202(+) (amiF) [Streptococcus mitis NCTC 12261]
MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSRE
QAAELIRRIQMIFQDPAASLNERATVDYIISEGLYNHHLFKDEEERKEKVKNIIREVGLLAEHLTRYPHEFSGGQRQRIG
IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGVIVEVAETEELFN
NPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHDYETDKPSMVEIRPGHYVWANQAELARYQQGLN

Nucleotide


Download         Length: 927 bp        

>NTDB_id=284628 SM12261_RS01530 WP_001291284.1 290276..291202(+) (amiF) [Streptococcus mitis NCTC 12261]
ATGTCTGAAAAATTAGTAGAAATCAAAGATTTAGAAATTTCCTTCGGTGAAGGAAGTAAGAAGTTTGTCGCAGTTAAAAA
TGCTAACTTCTTTATCAACAAGGGAGAAACTTTCTCGCTTGTAGGTGAGTCAGGTAGTGGGAAAACAACTATTGGTCGTG
CCATCATCGGTCTTAATGATACAAGTAATGGGGATATCATTTTTGATGGTCAAAAGATTAATGGTAAGAAATCGCGTGAA
CAAGCTGCAGAATTGATTCGTCGTATCCAGATGATTTTTCAAGATCCTGCCGCAAGTTTGAATGAACGTGCGACTGTTGA
TTATATTATTTCTGAAGGTCTTTACAATCATCATCTGTTCAAGGATGAAGAAGAACGTAAAGAGAAAGTTAAAAATATTA
TCCGTGAAGTGGGGCTTCTTGCTGAGCACTTGACTCGTTACCCTCATGAATTCTCAGGTGGTCAACGTCAACGTATCGGT
ATTGCCCGTGCCTTGGTCATGCAACCAGACTTTGTTATTGCGGATGAGCCCATTTCAGCCTTGGACGTTTCTGTGCGTGC
TCAAGTCTTGAACTTGCTCAAAAAATTCCAAAAAGAACTTGGTTTGACCTATCTCTTCATCGCCCATGACTTGTCTGTCG
TTCGCTTTATTTCAGATCGTATCGCAGTTATTTACAAGGGTGTTATTGTAGAGGTTGCAGAAACAGAAGAATTGTTTAAC
AATCCAATTCACCCATATACTCAAGCCTTGCTTTCAGCGGTACCAATCCCAGATCCAATCTTGGAACGTAAGAAGGTCTT
GAAAGTTTACGACCCAAGTCAACACGACTATGAGACTGATAAGCCATCTATGGTAGAAATCCGTCCAGGTCACTATGTTT
GGGCTAACCAAGCCGAATTGGCGCGTTACCAACAAGGGTTAAATTAA

Domains

Predicted by InterproScan.

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(230-262)

ABC transporter (ABC_tran)

(25-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8I8Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

85.902

99.026

0.851
  amiF Streptococcus salivarius strain HSISS4

85.902

99.026

0.851
  amiF Streptococcus thermophilus LMD-9

85.574

99.026

0.847

Multiple sequence alignment