Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGV95_RS31300 Genome accession   NZ_CP033684
Coordinates   6484262..6484885 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain H26027     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6479262..6489885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGV95_RS31280 (EGV95_31180) aroB 6479857..6480963 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  EGV95_RS31285 (EGV95_31185) aroK 6481013..6481531 (-) 519 WP_003095833.1 shikimate kinase AroK -
  EGV95_RS31290 (EGV95_31190) pilQ 6481543..6483687 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  EGV95_RS31295 (EGV95_31195) comL/pilP 6483741..6484265 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  EGV95_RS31300 (EGV95_31200) pilO 6484262..6484885 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGV95_RS31305 (EGV95_31205) pilN 6484882..6485478 (-) 597 WP_124014412.1 type 4a pilus biogenesis protein PilN Machinery gene
  EGV95_RS31310 (EGV95_31210) pilM 6485478..6486542 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  EGV95_RS31315 (EGV95_31215) - 6486727..6489198 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=284173 EGV95_RS31300 WP_003103268.1 6484262..6484885(-) (pilO) [Pseudomonas aeruginosa strain H26027]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=284173 EGV95_RS31300 WP_003103268.1 6484262..6484885(-) (pilO) [Pseudomonas aeruginosa strain H26027]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401