Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M30_RS18695 Genome accession   NZ_CP028218
Coordinates   3625584..3626201 (+) Length   205 a.a.
NCBI ID   WP_160244851.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102756     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620584..3631201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M30_RS18660 (C7M30_03701) aroE 3621242..3622084 (+) 843 WP_160244849.1 shikimate dehydrogenase -
  C7M30_RS18665 (C7M30_03702) yhbY 3622078..3622368 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C7M30_RS18670 (C7M30_03703) nadD 3622380..3622949 (+) 570 WP_014477393.1 nicotinate-nucleotide adenylyltransferase -
  C7M30_RS18675 (C7M30_03704) yqeK 3622939..3623499 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C7M30_RS18680 (C7M30_03705) rsfS 3623517..3623873 (+) 357 WP_014480315.1 ribosome silencing factor -
  C7M30_RS18685 (C7M30_03706) yqeM 3623870..3624613 (+) 744 WP_160244850.1 class I SAM-dependent methyltransferase -
  C7M30_RS18690 (C7M30_03707) comER 3624679..3625500 (-) 822 WP_014477389.1 late competence protein ComER -
  C7M30_RS18695 (C7M30_03708) comEA 3625584..3626201 (+) 618 WP_160244851.1 competence protein ComEA Machinery gene
  C7M30_RS18700 (C7M30_03709) comEB 3626268..3626837 (+) 570 WP_003229978.1 ComE operon protein 2 -
  C7M30_RS18705 (C7M30_03710) comEC 3626841..3629171 (+) 2331 WP_160244852.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M30_RS18710 (C7M30_03711) yqzM 3629211..3629345 (-) 135 WP_003229983.1 YqzM family protein -
  C7M30_RS18715 - 3629386..3629535 (+) 150 WP_160244853.1 hypothetical protein -
  C7M30_RS18720 (C7M30_03712) holA 3629575..3630618 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C7M30_RS18725 (C7M30_03713) rpsT 3630633..3630899 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21726.53 Da        Isoelectric Point: 4.7219

>NTDB_id=283454 C7M30_RS18695 WP_160244851.1 3625584..3626201(+) (comEA) [Bacillus subtilis strain SRCM102756]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMCT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVLGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=283454 C7M30_RS18695 WP_160244851.1 3625584..3626201(+) (comEA) [Bacillus subtilis strain SRCM102756]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATCATTTTGGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGTGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGATCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTTAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.909

100

0.385

Multiple sequence alignment