Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   D8668_RS02155 Genome accession   NZ_CP033439
Coordinates   472285..472908 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain SP4528     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467285..477908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8668_RS02140 (D8668_02140) - 467972..470443 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  D8668_RS02145 (D8668_02145) pilM 470628..471692 (+) 1065 WP_108720490.1 type IV pilus assembly protein PilM Machinery gene
  D8668_RS02150 (D8668_02150) pilN 471692..472288 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  D8668_RS02155 (D8668_02155) pilO 472285..472908 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  D8668_RS02160 (D8668_02160) comL/pilP 472905..473429 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  D8668_RS02165 (D8668_02165) pilQ 473483..475615 (+) 2133 WP_033989912.1 type 4a pilus secretin PilQ Machinery gene
  D8668_RS02170 (D8668_02170) aroK 475627..476145 (+) 519 WP_003095833.1 shikimate kinase AroK -
  D8668_RS02175 (D8668_02175) aroB 476195..477301 (+) 1107 WP_033989914.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=283403 D8668_RS02155 WP_003116135.1 472285..472908(+) (pilO) [Pseudomonas aeruginosa strain SP4528]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=283403 D8668_RS02155 WP_003116135.1 472285..472908(+) (pilO) [Pseudomonas aeruginosa strain SP4528]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396