Detailed information NTG0281048

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: C7S18_RS16650
• Genome accession: NZ_CP027860
• Coordinates: 4317670..4318728 (-)
• Length: 352 a.a.
• NCBI ID: WP_106892634.1
• Uniprot ID: A0A2P1PVA8
• Organism: Ahniella affigens strain D13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4312670..4323728

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7S18_RS16630 (C7S18_16615)	pilQ	4313595..4315814 (-)	2220	WP_106894071.1	type IV pilus secretin PilQ	-
C7S18_RS16635 (C7S18_16620)	-	4315828..4316370 (-)	543	WP_106892631.1	pilus assembly protein PilP	-
C7S18_RS16640 (C7S18_16625)	pilO	4316367..4317056 (-)	690	WP_106892632.1	type 4a pilus biogenesis protein PilO	-
C7S18_RS16645 (C7S18_16630)	-	4317056..4317670 (-)	615	WP_106892633.1	PilN domain-containing protein	-
C7S18_RS16650 (C7S18_16635)	pilM	4317670..4318728 (-)	1059	WP_106892634.1	pilus assembly protein PilM	Machinery gene
C7S18_RS16655 (C7S18_16640)	-	4319102..4321627 (+)	2526	WP_106892635.1	penicillin-binding protein 1A	-
C7S18_RS16660 (C7S18_16645)	-	4321596..4322237 (+)	642	WP_240623917.1	hypothetical protein	-
C7S18_RS16665 (C7S18_16650)	-	4322238..4322522 (-)	285	WP_206208045.1	acylphosphatase	-
C7S18_RS16670 (C7S18_16655)	-	4322528..4323019 (-)	492	WP_106892637.1	TlpA disulfide reductase family protein	-
C7S18_RS16675 (C7S18_16660)	wrbA	4323053..4323655 (+)	603	WP_240624053.1	NAD(P)H:quinone oxidoreductase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38064.89 Da
• Isoelectric Point: 4.4383

>NTDB_id=281048 C7S18_RS16650 WP_106892634.1 4317670..4318728(-) (pilM) [Ahniella affigens strain D13]
MALFSTKSPPLIGIDISSTAVKLLQLSQSGGRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRALQRSQTRLRHAAAA
VAGSAVITKTIPMPADLSEEDLEGAIQGEAQQYIPYPLEEVSMDFEVLGPVKDNPEMMNVMLAASRTENVELRVAALDLG
GLQAKVVDVEAFCMENAYKLVADQLSVPKDATVAVFDIGATMTTLIVLKNQRSIYTREQVFGGKQLTDEIMRRYGLSYEE
AGMAKRQGGLPESYEIEVLEPFKEAMVQQVSRLLQFFFAGTEYNKVDQIVLAGGCASIIGIAEMVEEQIGVPTVVANPLA
AMTLSSRVQAPAIAQDAPSLMIACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=281048 C7S18_RS16650 WP_106892634.1 4317670..4318728(-) (pilM) [Ahniella affigens strain D13]
GTGGCTCTCTTCAGTACAAAATCACCCCCGCTGATCGGCATCGACATCAGTTCGACAGCGGTGAAACTGCTCCAGCTCTC
CCAAAGTGGCGGGCGCTTTCGCGTGGAGCACTACGCCGTTGAACCACTGCCGCCCAATGCGGTGGTGGAGAAGAATATTG
TCGAAGTCGAAGCAGTCGGAGAAGCGATCAAGCGCGCCTTGCAGCGTTCGCAGACGCGTCTTCGTCATGCGGCGGCGGCT
GTCGCCGGTTCGGCAGTGATCACAAAAACCATCCCCATGCCCGCCGACCTGTCCGAAGAGGATCTGGAAGGCGCCATTCA
GGGTGAAGCGCAGCAATACATCCCGTACCCGCTCGAAGAAGTGAGTATGGACTTTGAGGTGCTCGGGCCGGTCAAAGACA
ACCCCGAAATGATGAACGTCATGCTGGCGGCATCGCGGACGGAAAATGTCGAGCTTCGGGTTGCCGCGCTGGACTTGGGC
GGTTTGCAGGCAAAAGTCGTCGATGTTGAAGCCTTTTGCATGGAAAACGCTTACAAGTTGGTTGCTGACCAACTGAGCGT
CCCGAAGGACGCGACGGTTGCCGTGTTCGACATCGGCGCGACCATGACCACGCTGATCGTGCTGAAGAATCAGCGCAGCA
TTTATACGCGGGAGCAGGTTTTCGGCGGCAAGCAGCTGACGGACGAGATCATGCGTCGCTACGGCTTGTCGTACGAAGAA
GCCGGTATGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGTTACGAAATCGAAGTGCTCGAACCGTTCAAGGAAGCCATGGT
GCAGCAAGTCAGTCGTCTGCTGCAGTTCTTCTTTGCCGGCACGGAATACAACAAGGTTGACCAAATTGTGCTTGCCGGTG
GTTGCGCATCCATCATCGGCATCGCCGAAATGGTTGAAGAGCAGATCGGCGTGCCGACCGTGGTCGCGAACCCGCTCGCG
GCGATGACCTTGTCCAGTCGGGTGCAGGCGCCTGCGATCGCACAGGACGCGCCGTCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-351)

3D structure

• AlphaFold DB: A0A2P1PVA8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	52.557	100	0.526
comM	Acinetobacter nosocomialis M2	52.273	100	0.523
comM	Acinetobacter baylyi ADP1	48.864	100	0.489
pilM	Legionella pneumophila strain ERS1305867	42.776	100	0.429