Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K20_RS02285 Genome accession   NZ_CP027743
Coordinates   506551..507603 (+) Length   350 a.a.
NCBI ID   WP_124298332.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain CW2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501551..512603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K20_RS02275 (C4K20_0455) - 502503..503771 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K20_RS02280 (C4K20_0456) - 503899..506334 (-) 2436 WP_164485973.1 penicillin-binding protein 1A -
  C4K20_RS02285 (C4K20_0458) pilM 506551..507603 (+) 1053 WP_124298332.1 pilus assembly protein PilM Machinery gene
  C4K20_RS02290 (C4K20_0459) - 507603..508169 (+) 567 WP_124298333.1 PilN domain-containing protein -
  C4K20_RS02295 (C4K20_0460) pilO 508166..508783 (+) 618 WP_124298334.1 type 4a pilus biogenesis protein PilO -
  C4K20_RS02300 (C4K20_0461) - 508780..509307 (+) 528 WP_124298335.1 pilus assembly protein PilP -
  C4K20_RS02305 (C4K20_0462) pilQ 509321..511393 (+) 2073 WP_124298336.1 type IV pilus secretin PilQ Machinery gene
  C4K20_RS02310 (C4K20_0463) aroK 511398..511916 (+) 519 WP_124298337.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37103.62 Da        Isoelectric Point: 5.0008

>NTDB_id=279882 C4K20_RS02285 WP_124298332.1 506551..507603(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain CW2]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALVAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALVSEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279882 C4K20_RS02285 WP_124298332.1 506551..507603(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain CW2]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGACCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGTGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGTCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment