Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K22_RS02310 Genome accession   NZ_CP027741
Coordinates   510511..511563 (+) Length   350 a.a.
NCBI ID   WP_028683780.1    Uniprot ID   A0AAJ0ZMY7
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain 449     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505511..516563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K22_RS02300 (C4K22_0463) - 506461..507729 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K22_RS02305 (C4K22_0464) - 507859..510294 (-) 2436 WP_164485365.1 penicillin-binding protein 1A -
  C4K22_RS02310 (C4K22_0466) pilM 510511..511563 (+) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene
  C4K22_RS02315 (C4K22_0467) - 511563..512129 (+) 567 WP_124333889.1 PilN domain-containing protein -
  C4K22_RS02320 (C4K22_0468) pilO 512126..512743 (+) 618 WP_124333890.1 type 4a pilus biogenesis protein PilO -
  C4K22_RS02325 (C4K22_0469) - 512740..513267 (+) 528 WP_124333891.1 pilus assembly protein PilP -
  C4K22_RS02330 (C4K22_0470) pilQ 513281..515353 (+) 2073 WP_124333892.1 type IV pilus secretin PilQ Machinery gene
  C4K22_RS02335 (C4K22_0471) aroK 515358..515876 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37047.51 Da        Isoelectric Point: 5.0008

>NTDB_id=279828 C4K22_RS02310 WP_028683780.1 510511..511563(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain 449]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279828 C4K22_RS02310 WP_028683780.1 510511..511563(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain 449]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGTGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGACCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAAATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment