Detailed information NTG0027981

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PST_RS19730
• Genome accession: NC_009434
• Coordinates: 4300655..4301800 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri A1501
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4295655..4306800

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PST_RS19705 (PST_3959)	-	4295732..4296748 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
PST_RS19710 (PST_3960)	-	4296748..4298022 (+)	1275	WP_011914965.1	dihydroorotase	-
PST_RS19715 (PST_3961)	-	4298080..4298475 (-)	396	WP_011914966.1	NINE protein	-
PST_RS19720 (PST_3962)	-	4298611..4299825 (+)	1215	WP_041755962.1	putative DNA modification/repair radical SAM protein	-
PST_RS19725 (PST_3963)	-	4299825..4300658 (+)	834	WP_011914968.1	TIGR03915 family putative DNA repair protein	-
PST_RS19730 (PST_3964)	pilU	4300655..4301800 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PST_RS19735 (PST_3965)	pilT	4301860..4302894 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
PST_RS19740 (PST_3966)	-	4302961..4303650 (+)	690	WP_011914971.1	YggS family pyridoxal phosphate-dependent enzyme	-
PST_RS19745 (PST_3967)	proC	4303661..4304485 (+)	825	WP_041755828.1	pyrroline-5-carboxylate reductase	-
PST_RS19750 (PST_3968)	-	4304495..4305085 (+)	591	WP_011914973.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=27981 PST_RS19730 WP_019405534.1 4300655..4301800(-) (pilU) [Stutzerimonas stutzeri A1501]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=27981 PST_RS19730 WP_019405534.1 4300655..4301800(-) (pilU) [Stutzerimonas stutzeri A1501]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCATC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391