Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K23_RS02175 Genome accession   NZ_CP027716
Coordinates   483386..484438 (+) Length   350 a.a.
NCBI ID   WP_023970126.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain Pb-St2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478386..489438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K23_RS02165 (C4K23_0444) - 479298..480566 (+) 1269 WP_023970124.1 malic enzyme-like NAD(P)-binding protein -
  C4K23_RS02170 (C4K23_0445) - 480734..483169 (-) 2436 WP_193725266.1 penicillin-binding protein 1A -
  C4K23_RS02175 (C4K23_0447) pilM 483386..484438 (+) 1053 WP_023970126.1 pilus assembly protein PilM Machinery gene
  C4K23_RS02180 (C4K23_0448) - 484438..485004 (+) 567 WP_023970127.1 PilN domain-containing protein -
  C4K23_RS02185 (C4K23_0449) pilO 485001..485618 (+) 618 WP_023970128.1 type 4a pilus biogenesis protein PilO -
  C4K23_RS02190 (C4K23_0450) - 485615..486142 (+) 528 WP_023970129.1 pilus assembly protein PilP -
  C4K23_RS02195 (C4K23_0451) pilQ 486156..488228 (+) 2073 WP_023970130.1 type IV pilus secretin PilQ Machinery gene
  C4K23_RS02200 (C4K23_0452) aroK 488233..488751 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37174.65 Da        Isoelectric Point: 5.0088

>NTDB_id=279194 C4K23_RS02175 WP_023970126.1 483386..484438(+) (pilM) [Pseudomonas chlororaphis strain Pb-St2]
MLGLFSKKAGSLLGIDISSTSVKLLELSRVGDRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHERQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279194 C4K23_RS02175 WP_023970126.1 483386..484438(+) (pilM) [Pseudomonas chlororaphis strain Pb-St2]
GTGCTAGGACTCTTCAGTAAAAAGGCGGGCTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGACCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAGCGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAACTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTCGTGCATGAAAGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAGGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTACG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.83

100

0.551
  comM Acinetobacter nosocomialis M2

54.545

100

0.549
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment