Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K36_RS02230 Genome accession   NZ_CP027709
Coordinates   497126..498178 (+) Length   350 a.a.
NCBI ID   WP_124323331.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain ATCC 17809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492126..503178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K36_RS02220 (C4K36_0450) - 493038..494306 (+) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -
  C4K36_RS02225 (C4K36_0451) - 494474..496909 (-) 2436 WP_164486060.1 penicillin-binding protein 1A -
  C4K36_RS02230 (C4K36_0453) pilM 497126..498178 (+) 1053 WP_124323331.1 pilus assembly protein PilM Machinery gene
  C4K36_RS02235 (C4K36_0454) - 498178..498744 (+) 567 WP_025808191.1 PilN domain-containing protein -
  C4K36_RS02240 (C4K36_0455) pilO 498741..499358 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  C4K36_RS02245 (C4K36_0456) - 499355..499882 (+) 528 WP_124323332.1 pilus assembly protein PilP -
  C4K36_RS02250 (C4K36_0457) pilQ 499896..501968 (+) 2073 WP_124323333.1 type IV pilus secretin PilQ Machinery gene
  C4K36_RS02255 (C4K36_0458) aroK 501973..502491 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37231.70 Da        Isoelectric Point: 5.1221

>NTDB_id=279011 C4K36_RS02230 WP_124323331.1 497126..498178(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain ATCC 17809]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARTVALIDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYRLSKEEAG
LVKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLSLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279011 C4K36_RS02230 WP_124323331.1 497126..498178(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain ATCC 17809]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGTTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAACCCCGAGCGGGTCGATGTGCTGTTGGCGGCTTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGACCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGCATCAGTTGACCGAGCACATCCAGCGTCGTTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGTGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGGGGTAGCG
CCTCGGTTCCAGGGCTTGCACCGTTGATCGAGCAACGCCTGAGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCCCCGTCCCTGATGATCGCTTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.261

100

0.546
  comM Acinetobacter nosocomialis M2

53.977

100

0.543
  comM Acinetobacter baylyi ADP1

51.42

100

0.517
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454

Multiple sequence alignment