Detailed information NTG0278481

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AM480_RS04690
• Genome accession: NZ_CP027611
• Coordinates: 944444..945502 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AR_0101
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 939444..950502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM480_RS04665 (AM480_04665)	aroK	939719..940288 (-)	570	WP_000643712.1	shikimate kinase AroK	-
AM480_RS04670 (AM480_04670)	pilQ	940300..942465 (-)	2166	WP_001017038.1	type IV pilus secretin PilQ family protein	Machinery gene
AM480_RS04675 (AM480_04675)	pilP	942528..943055 (-)	528	WP_000695058.1	pilus assembly protein PilP	Machinery gene
AM480_RS04680 (AM480_04680)	pilO	943066..943806 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
AM480_RS04685 (AM480_04685)	pilN	943803..944444 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
AM480_RS04690 (AM480_04690)	pilM	944444..945502 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
AM480_RS04695 (AM480_04695)	ponA	945664..948219 (+)	2556	WP_000736683.1	penicillin-binding protein PBP1a	-
AM480_RS04700 (AM480_04700)	-	948237..949061 (-)	825	WP_086242007.1	putative RNA methyltransferase	-
AM480_RS04705 (AM480_04705)	-	949064..949396 (-)	333	WP_000169601.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=278481 AM480_RS04690 WP_002014066.1 944444..945502(-) (pilM) [Acinetobacter baumannii strain AR_0101]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=278481 AM480_RS04690 WP_002014066.1 944444..945502(-) (pilM) [Acinetobacter baumannii strain AR_0101]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAATGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCGGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCCTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACTATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCCTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423