Detailed information NTG0277003

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: CFN54_RS01980
• Genome accession: NZ_CP032635
• Coordinates: 439332..440339 (+)
• Length: 335 a.a.
• NCBI ID: WP_016264643.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain ZFM225
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 434332..445339

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFN54_RS01960 (CFN54_01960)	hexB	435375..437336 (+)	1962	WP_035146236.1	DNA mismatch repair endonuclease MutL	Machinery gene
CFN54_RS01965 (CFN54_01965)	-	437336..437881 (+)	546	WP_016264640.1	Maf family protein	-
CFN54_RS01970 (CFN54_01970)	-	437894..438457 (+)	564	WP_016264641.1	TIGR00730 family Rossman fold protein	-
CFN54_RS01975 (CFN54_01975)	ruvA	438708..439319 (+)	612	WP_016264642.1	Holliday junction branch migration protein RuvA	Machinery gene
CFN54_RS01980 (CFN54_01980)	ruvB	439332..440339 (+)	1008	WP_016264643.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
CFN54_RS01985 (CFN54_01985)	queA	440354..441385 (+)	1032	WP_011374077.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
CFN54_RS01990 (CFN54_01990)	-	441412..441933 (+)	522	WP_016264644.1	GNAT family N-acetyltransferase	-
CFN54_RS01995 (CFN54_01995)	arcA	442141..443376 (+)	1236	WP_016264645.1	arginine deiminase	-
CFN54_RS02000 (CFN54_02000)	argF	443402..444418 (+)	1017	WP_011374080.1	ornithine carbamoyltransferase	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 37247.68 Da
• Isoelectric Point: 4.8413

>NTDB_id=277003 CFN54_RS01980 WP_016264643.1 439332..440339(+) (ruvB) [Latilactobacillus sakei strain ZFM225]
MADERIVSAENDDFAEASIEKTLRPQVLAQYIGQDRVKNELAVYIEAAKKREESLDHVLLYGPPGLGKTTLAMVIANELQ
VQIRTTSGPAIERPGDLVALLNELQPGDVLFIDEIHRLPKMVEELLYSAMEDFYIDIVVGQGPTAHPVHFPLPPFTLIGA
TTRAGLLSAPLRDRFGIVEHMAYYTEADLMDIVQRSAGVFNMSIVPDGALEIARRSRGTPRIANRLLKRTRDYAQVADQN
TIDQAIADHALSQLQVDIRGLDGVDRKILQMMIDYYQGGPVGLKTIAANIGEENETIEEVYEPYLLQIGFLKRTQRGRLV
TPAGYAHLGMPYPEK

Nucleotide

• Length: 1008 bp

>NTDB_id=277003 CFN54_RS01980 WP_016264643.1 439332..440339(+) (ruvB) [Latilactobacillus sakei strain ZFM225]
ATGGCAGATGAGCGAATTGTTTCAGCCGAAAACGATGATTTTGCTGAAGCAAGCATTGAAAAAACATTGCGGCCACAAGT
CCTTGCACAATATATCGGTCAAGATCGGGTTAAAAATGAATTAGCGGTCTATATTGAAGCGGCCAAAAAACGGGAAGAGT
CCCTCGATCACGTTTTACTTTATGGCCCACCAGGTTTGGGGAAAACCACGTTAGCGATGGTGATTGCCAACGAGTTGCAA
GTCCAGATTCGAACGACAAGTGGCCCCGCAATCGAGCGGCCTGGAGATTTAGTGGCGTTATTAAACGAGTTACAACCTGG
GGATGTTTTATTTATTGATGAAATTCATCGCTTACCTAAGATGGTTGAAGAATTACTTTATTCAGCGATGGAAGATTTCT
ACATCGATATTGTGGTTGGTCAAGGGCCTACCGCACATCCCGTCCATTTTCCATTACCACCATTCACATTGATTGGCGCG
ACGACGCGGGCGGGCTTATTGTCTGCGCCATTGCGAGATCGTTTCGGGATTGTGGAACACATGGCTTATTACACGGAGGC
TGATTTGATGGACATTGTGCAACGCTCAGCCGGCGTCTTTAATATGTCGATTGTGCCAGATGGTGCGTTAGAAATCGCAC
GTCGCTCGCGAGGGACGCCGCGGATTGCCAATCGGTTGTTGAAACGAACTCGAGATTACGCACAAGTGGCTGACCAAAAT
ACGATTGATCAGGCGATTGCTGATCACGCATTAAGTCAGCTGCAAGTTGATATTCGGGGCTTAGACGGTGTCGATCGCAA
AATCCTCCAGATGATGATTGATTATTATCAAGGCGGACCAGTCGGTTTAAAAACCATTGCCGCCAATATTGGTGAAGAAA
ACGAAACAATTGAAGAAGTTTACGAGCCTTATTTACTGCAAATTGGCTTTTTAAAACGAACGCAAAGAGGCCGGCTGGTA
ACGCCGGCTGGTTATGCCCATTTAGGCATGCCTTATCCAGAAAAATAG

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (260-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	64.653	98.806	0.639
ruvB	Streptococcus pneumoniae R6	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae D39	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae TIGR4	64.375	95.522	0.615
ruvB	Synechocystis sp. PCC 6803	58.28	93.731	0.546
ruvB	Helicobacter pylori 26695	51.829	97.91	0.507