Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLWM1_RS11645 Genome accession   NZ_CP032500
Coordinates   2253043..2254116 (-) Length   357 a.a.
NCBI ID   WP_014570824.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain WM1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2248043..2259116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLWM1_RS11600 (LLWM1_03195) - 2248234..2248356 (-) 123 WP_023164646.1 hypothetical protein -
  LLWM1_RS11605 (LLWM1_2221) - 2248353..2248535 (-) 183 WP_003130605.1 hypothetical protein -
  LLWM1_RS11610 (LLWM1_2222) - 2248551..2249240 (-) 690 WP_058221713.1 Rha family transcriptional regulator -
  LLWM1_RS11615 (LLWM1_2223) - 2249299..2249532 (-) 234 WP_014570819.1 helix-turn-helix transcriptional regulator -
  LLWM1_RS11620 (LLWM1_2224) - 2249709..2250119 (+) 411 WP_014570820.1 helix-turn-helix domain-containing protein -
  LLWM1_RS11625 (LLWM1_2225) - 2250130..2250714 (+) 585 WP_058221714.1 hypothetical protein -
  LLWM1_RS11630 (LLWM1_2226) - 2250770..2251309 (+) 540 WP_023189015.1 PH domain-containing protein -
  LLWM1_RS11635 (LLWM1_2227) - 2251435..2252892 (+) 1458 WP_058221720.1 recombinase family protein -
  LLWM1_RS11640 (LLWM1_03200) - 2252889..2253029 (-) 141 WP_023164653.1 hypothetical protein -
  LLWM1_RS11645 (LLWM1_2228) comGB 2253043..2254116 (-) 1074 WP_014570824.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLWM1_RS11650 (LLWM1_2229) comGA 2254010..2254949 (-) 940 Protein_2268 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40366.59 Da        Isoelectric Point: 10.4062

>NTDB_id=276415 LLWM1_RS11645 WP_014570824.1 2253043..2254116(-) (comGB) [Lactococcus lactis subsp. lactis strain WM1]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=276415 LLWM1_RS11645 WP_014570824.1 2253043..2254116(-) (comGB) [Lactococcus lactis subsp. lactis strain WM1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.698

95.518

0.79
  comGB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comGB Streptococcus mutans UA140

53.372

95.518

0.51
  comGB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae D39

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae R6

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

93.838

0.482
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471