Detailed information NTG0275566

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CSB68_RS13290
• Genome accession: NZ_CP027183
• Coordinates: 2696837..2697955 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AR_0052
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2691837..2702955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CSB68_RS13280 (CSB68_2663)	-	2694961..2695653 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
CSB68_RS13285 (CSB68_2664)	pilT	2695780..2696817 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CSB68_RS13290 (CSB68_2665)	pilU	2696837..2697955 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CSB68_RS13295 (CSB68_2666)	fur	2698025..2698462 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CSB68_RS13300 (CSB68_2667)	-	2698574..2698972 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CSB68_RS13305 (CSB68_2668)	-	2699027..2699350 (-)	324	WP_000442589.1	RnfH family protein	-
CSB68_RS13310 (CSB68_2669)	-	2699350..2700417 (-)	1068	WP_029777448.1	membrane protein	-
CSB68_RS13320 (CSB68_2670)	-	2700570..2701025 (-)	456	WP_000782976.1	bacteriohemerythrin	-
CSB68_RS13325 (CSB68_2671)	-	2701278..2702126 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=275566 CSB68_RS13290 WP_000347036.1 2696837..2697955(+) (pilU) [Acinetobacter baumannii strain AR_0052]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=275566 CSB68_RS13290 WP_000347036.1 2696837..2697955(+) (pilU) [Acinetobacter baumannii strain AR_0052]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392