Detailed information NTG0275516

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CSB70_RS02815
• Genome accession: NZ_CP027178
• Coordinates: 585507..586625 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AR_0070
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 580507..591625

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CSB70_RS02780 (CSB70_0557)	-	581336..582184 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
CSB70_RS02785 (CSB70_0558)	-	582437..582892 (+)	456	WP_000782976.1	bacteriohemerythrin	-
CSB70_RS02795 (CSB70_0559)	-	583045..584112 (+)	1068	WP_029777448.1	membrane protein	-
CSB70_RS02800 (CSB70_0560)	-	584112..584435 (+)	324	WP_000442589.1	RnfH family protein	-
CSB70_RS02805 (CSB70_0561)	-	584490..584888 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CSB70_RS02810 (CSB70_0562)	fur	585000..585437 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CSB70_RS02815 (CSB70_0563)	pilU	585507..586625 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CSB70_RS02820 (CSB70_0564)	pilT	586645..587682 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CSB70_RS02825 (CSB70_0565)	-	587809..588501 (+)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=275516 CSB70_RS02815 WP_000347036.1 585507..586625(-) (pilU) [Acinetobacter baumannii strain AR_0070]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=275516 CSB70_RS02815 WP_000347036.1 585507..586625(-) (pilU) [Acinetobacter baumannii strain AR_0070]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392